Clin Exp Reprod Med.  2016 Dec;43(4):193-198. 10.5653/cerm.2016.43.4.193.

Glucose-6-phosphate dehydrogenase deficiency does not increase the susceptibility of sperm to oxidative stress induced by Hâ‚‚Oâ‚‚

• Roshankhah S
• Rostami-Far Z
• Shaveisi-Zadeh F
• Movafagh A
• Bakhtiari
• Shaveisi-Zadeh J

• KMID: 2364655
• DOI: http://doi.org/10.5653/cerm.2016.43.4.193

Abstract

OBJECTIVE
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect. G6PD plays a key role in the pentose phosphate pathway, which is a major source of nicotinamide adenine dinucleotide phosphate (NADPH). NADPH provides the reducing equivalents for oxidation-reduction reductions involved in protecting against the toxicity of reactive oxygen species such as Hâ‚‚Oâ‚‚. We hypothesized that G6PD deficiency may reduce the amount of NADPH in sperms, thereby inhibiting the detoxification of Hâ‚‚Oâ‚‚, which could potentially affect their motility and viability, resulting in an increased susceptibility to infertility.
METHODS
Semen samples were obtained from four males with G6PD deficiency and eight healthy males as a control. In both groups, motile sperms were isolated from the seminal fluid and incubated with 0, 10, 20, 40, 60, 80, and 120 µM concentrations of H2O2. After 1 hour incubation at 37℃, sperms were evaluated for motility and viability.
RESULTS
Incubation of sperms with 10 and 20 µM Hâ‚‚Oâ‚‚ led to very little decrease in motility and viability, but motility decreased notably in both groups in 40, 60, and 80 µM Hâ‚‚Oâ‚‚, and viability decreased in both groups in 40, 60, 80, and 120 µM Hâ‚‚Oâ‚‚. However, no statistically significant differences were found between the G6PD-deficient group and controls.
CONCLUSION
G6PD deficiency does not increase the susceptibility of sperm to oxidative stress induced by Hâ‚‚Oâ‚‚, and the reducing equivalents necessary for protection against Hâ‚‚Oâ‚‚ are most likely produced by other pathways. Therefore, G6PD deficiency cannot be considered as major risk factor for male infertility.

Keyword

Glucose-6-phosphate dehydrogenase deficiency; Infertility; Oxidative stress; Risk factors

MeSH Terms

Glucose-6-Phosphate*
Glucosephosphate Dehydrogenase Deficiency*
Glucosephosphate Dehydrogenase*
Humans
Infertility
Infertility, Male
Male
NADP
Oxidation-Reduction
Oxidative Stress*
Pentose Phosphate Pathway
Reactive Oxygen Species
Risk Factors
Semen
Spermatozoa*
Glucose-6-Phosphate
Glucosephosphate Dehydrogenase
NADP
Reactive Oxygen Species