Korean J Physiol Pharmacol.  2016 Nov;20(6):565-571. 10.4196/kjpp.2016.20.6.565.

The effects of paeoniflorin injection on soluble triggering receptor expressed on myeloid-1 (sTREM-1) levels in severe septic rats

• Liu XR
• Xu J
• Wang YM
• Ji MS
• Liu FS

• KMID: 2364260
• DOI: http://doi.org/10.4196/kjpp.2016.20.6.565

Abstract

Paeoniflorin (PAE) is the most abundant compound in Xuebijing injection widely used to treat sepsis. We aimed to investigate effect of PAE on expression of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in a rat model of sepsis. Wistar rats were divided into Normal, Model, and PAE groups (n=20 each). Endotoxin was administrated at 5 mg/ml/kg in Model and PAE rats to establish rat sepsis model. 1 h after endotoxin administration, PAE was administrated at 4 ml/kg in PAE group once per day for 3 days. Routine blood tests and biochemical indexes were assessed, including aspartate aminotransferase (AST) and creatine kinase-MB (CK-MB). The plasma sTREM-1 level was measured using quantitative ELISA. At the end of experiment, the small intestine, liver, kidney and lung were subjected to pathological examinations. A rat model of sepsis-induced multiple organ dysfunction syndrome (MODS) was established successfully with endotoxin administration (5 mg/ml/kg), evidenced by histo-pathological examinations, routine blood tests and biochemical indexes: platelet count decreased and white blood cell count increased (p<0.05), CK-MB and AST increased (p<0.05). PAE treatment significantly reduced the plasma levels of AST, CK-MB, and sTREM-1, compared to Model group (p<0.05). Meanwhile, sepsis-induced damages in the liver, lung, stomach and intestinal mucosa were also markedly ameliorated by PAE treatment. PAE demonstrated a significantly protective effect in a rat model of sepsis by decreasing plasma sTREM-1 level, reducing inflammation, preventing MODS and protecting organ functions.

Keyword

Dysfunction syndrome; Endotoxin; Paeoniflorin; Sepsis; Soluble TREM-1; Xuebijing

MeSH Terms

Animals
Aspartate Aminotransferases
Creatine
Enzyme-Linked Immunosorbent Assay
Hematologic Tests
Inflammation
Intestinal Mucosa
Intestine, Small
Kidney
Leukocyte Count
Liver
Lung
Models, Animal
Multiple Organ Failure
Plasma
Platelet Count
Rats*
Rats, Wistar
Sepsis
Stomach
Aspartate Aminotransferases
Creatine

Figure

• 

  Fig. 1 Platelet (A) and white blood cell (B) count of rats in PAE, Model and Normal experimental groups (see Materials and Methods section) at indicated time points.Data are shown as mean±SEM (n=20). *p<0.05 PAE and Model vs Normal; **p<0.05 PAE vs either Model or Normal; #p<0.01 PAE vs Model.

• 

  Fig. 2 CK-MB (A) and AST (B) test of rats in PAE, Model and Normal experimental groups (see Materials and Methods section) at indicated time points. Data are shown as mean±SEM (n=20). *p<0.05 PAE and Model vs Normal; **p<0.05 PAE vs either Model or Normal; #p<0.05 PAE vs Model.

• 

  Fig. 3 Relative plasma sTREM-1 levels of rats in PAE, Model and Normal experimental groups (see Materials and Methods section) at indicated time points.Data are shown as mean±SEM (n=20). *p<0.05 PAE and Model vs Normal; **p<0.05 PAE vs either Model or Normal; #p<0.01 PAE vs Model.

• 

  Fig. 4 Histo-pathology of organs from rats in PAE, Model and Normal experimental groups

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