Ann Lab Med.  2015 Mar;35(2):263-265. 10.3343/alm.2015.35.2.263.

Efficacy of the Measurement of 25-Hydroxyvitamin D2 and D3 Levels by Using PerkinElmer Liquid Chromatography-Tandem Mass Spectrometry Vitamin D Kit Compared With DiaSorin Radioimmunoassay Kit and Elecsys Vitamin D Total Assay

• Kwak HS
• Chung HJ
• Cho DH
• Park MH
• Ku ES
• Park EJ
• Oh HJ

• KMID: 2363192
• DOI: http://doi.org/10.3343/alm.2015.35.2.263

Abstract

No abstract available.

MeSH Terms

25-Hydroxyvitamin D 2/*blood
Cholecalciferol/*blood
Chromatography, High Pressure Liquid
Humans
*Radioimmunoassay
Reagent Kits, Diagnostic
*Tandem Mass Spectrometry
25-Hydroxyvitamin D 2
Cholecalciferol
Reagent Kits, Diagnostic

Figure

• 

  Fig. 1 Comparison between immunometric assays and LC-MS/MS (PerkinElmer MS/MS Vitamin D kit) for 25-hydroxyvitamin D quantification: (A, B) Linear regression between LC-MS/MS and ECLIA (Elecsys Vitamin D total assay), and LC-MS/MS and RIA (DiaSorin RIA kit), respectively. (C, D) Bland-Altman plot between LC-MS/MS and ECLIA, and LC-MS/MS and RIA, respectively. Open circles represent samples containing relatively low concentrations of 25-hydroxyvitamin D2 (<1 ng/mL), and black circles represent samples containing relatively high concentrations of 25-hydroxyvitamin D2 (≥1 ng/mL).Abbreviations: ECLIA, electrochemiluminescence immunoassay; LC-MS/MS, liquid chromatography-tandem mass spectrometry.

• 

  Fig. 2 Distribution for 25-hydroxyvitamin D3 (25OHD3) (A) and 25-hydroxyvitamin D2 (25OHD2) (B). Results were obtained by analyzing serum samples provided by 50 volunteers.

Reference

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