Ann Lab Med.  2015 Mar;35(2):250-253. 10.3343/alm.2015.35.2.250.

Clinical and Microbiological Characteristics of Six Staphylococcus pettenkoferi Isolates From Blood Samples

Affiliations
  • 1Department of Laboratory Medicine, Ewha Womans University School of Medicine, Seoul, Korea. miae@ewha.ac.kr, sunny0521.chung@gmail.com

Abstract

Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood specimens, including one pathogen and 5 contaminants and catheter colonizers. Brucker Biotyper (Brucker Daltonics, Bremen, Germany) and molecular typing with 16S rRNA gene sequencing confirmed the 6 isolates as S. pettenkoferi. The conventional phenotypic identification of these isolates is not reliable owing to their inconsistent biochemical characteristics. Five of the 6 isolates were found to be resistant to oxacillin, and all isolates showed susceptibility to vancomycin and linezolid. For accurate identification of this novel species, advanced methods by using Brucker Biotyper or molecular methods such as 16S rRNA gene sequencing are required.

Keyword

Staphylococcus pettenkoferi; MALDI-TOF MS; 16S ribosomal RNA

MeSH Terms

Aged
Aged, 80 and over
Anti-Bacterial Agents/pharmacology/therapeutic use
DNA, Bacterial/chemistry/metabolism
Drug Resistance, Bacterial/drug effects
Female
Humans
Linezolid/pharmacology
Male
Microbial Sensitivity Tests
Middle Aged
Oxacillin/pharmacology
Phenotype
RNA, Ribosomal, 16S/chemistry/genetics/metabolism
Sequence Analysis, DNA
Staphylococcal Infections/drug therapy/*microbiology/pathology
Staphylococcus/drug effects/*genetics/isolation & purification
Vancomycin/pharmacology
Anti-Bacterial Agents
DNA, Bacterial
Linezolid
Oxacillin
RNA, Ribosomal, 16S
Vancomycin
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