Ann Lab Med.  2015 Jan;35(1):123-127. 10.3343/alm.2015.35.1.123.

Performance of LIFECODES HLA-DQB1 Typing Kit Using Luminex Platform in Koreans

• Roh EY
• In JW
• Shin S
• Yoon JH
• Park KU
• Song EY

• KMID: 2363159
• DOI: http://doi.org/10.3343/alm.2015.35.1.123

Abstract

Intermediate-resolution HLA-DQ typing has gained importance in organ transplantation recently. We evaluated the performance of the LIFECODES HLA-DQB1 typing kit (Immucor, USA) using sequence-specific oligonucleotide (SSO) probe and Luminex platform (Luminex Corp., USA) on 100 samples tested by sequence-based typing (SBT) using the AlleleSEQR HLA-DQB1 kit (Abbott Molecular, USA) in Korean individuals. No sample showed ambiguity in the assignment of 4-digit HLA-DQB1 allele with the LIFECODES HLA-DQB1 SSO typing kit, and the results were fully concordant with those of high-resolution typing of AlleleSEQR HLA-DQB1 SBT up to 4-digit level. Three samples required adjustment of false reactions (3/100, 3.0%): two samples with DQB1*03:03/*06:01 showed false-positive result in probe 253, and 1 sample with DQB1*04:02/*05:02 showed false-negative result in probe 217. We tested an additional sample with DQB1*03:03/*06:01, which showed same false-positivity in probe 253 and 2 samples with DQB1*04:02/*05:02, which showed no false reaction. The false reactions did not result in ambiguity or change in the HLA allele assignment. We could assign HLA-DQB1 alleles to 4 digit-level without ambiguity, with 100% concordance with the SBT results. Thus, LIFECODES HLA-DQB1 SSO typing kit showed good performance for intermediate-resolution HLA-DQB1 typing in clinical laboratory for organ transplantation in Koreans.

Keyword

HLA-DQB1; PCR-SSO; Luminex; Korean; Organ transplantation

MeSH Terms

Alleles
DNA Primers/metabolism
Gene Frequency
Genotype
HLA-DQ beta-Chains/*genetics/metabolism
Histocompatibility Testing/*standards
Humans
Polymerase Chain Reaction
Reagent Kits, Diagnostic/*standards
Republic of Korea
DNA Primers
HLA-DQ beta-Chains
HLA-DQB1 antigen
Reagent Kits, Diagnostic

Reference

1. Tambur AR, Rosati J, Roitberg S, Glotz D, Friedewald JJ, Leventhal JR. Epitope analysis of HLA-DQ antigens: what does the antibody see? Transplantation. 2014; 98:157–166. PMID: 25003284.

2. Lachmann N, Todorova K, Schulze H, Schönemann C. Luminex® and its applications for solid organ transplantation, hematopoietic stem cell transplantation, and transfusion. Transfus Med Hemother. 2013; 40:182–189. PMID: 23922543.

3. Trajanoski D, Fidler SJ. HLA typing using bead-based methods. Methods Mol Biol. 2012; 882:47–65. PMID: 22665228.

4. Testi M, Iannelli S, Testa G, Troiano M, Capelli S, Fruet F, et al. Evaluation of DRB1 high resolution typing by a new SSO-based Luminex method. Mol Biol Rep. 2012; 39:13–16. PMID: 21424786.

5. Song EY, Park MH, Kang SJ, Park HJ, Kim BC, Tokunaga K, et al. HLA class II allele and haplotype frequencies in Koreans based on 107 families. Tissue Antigens. 2002; 59:475–486. PMID: 12445317.

6. Roh EY, Park MH, Park H, Park DH, Choi JB, Kim SJ, et al. Association of HLA-DR and -DQ genes with narcolepsy in Koreans: comparison with two control groups, randomly selected subjects and DRB1^*1501-DQB1^*0602--positive subjects. Hum Immunol. 2006; 67:749–755. PMID: 17002906.

7. DeVos JM, Gaber AO, Knight RJ, Land GA, Suki WN, Gaber LW, et al. Donor-specific HLA-DQ antibodies may contribute to poor graft outcome after renal transplantation. Kidney Int. 2012; 82:598–604. PMID: 22622504.

8. Willicombe M, Brookes P, Sergeant R, Santos-Nunez E, Steggar C, Galliford J, et al. De novo DQ donor-specific antibodies are associated with a significant risk of antibody-mediated rejection and transplant glomerulopathy. Transplantation. 2012; 94:172–177. PMID: 22735711.

9. Tambur AR, Leventhal JR, Friedewald JJ, Ramon DS. The complexity of human leukocyte antigen (HLA)-DQ antibodies and its effect on virtual crossmatching. Transplantation. 2010; 90:1117–1124. PMID: 20847715.

10. Claas FH, Rahmel A, Doxiadis II. Enhanced kidney allocation to highly sensitized patients by the acceptable mismatch program. Transplantation. 2009; 88:447–452. PMID: 19696624.

11. Saito S, Ota S, Yamada E, Inoki H, Ota M. Allele frequencies and haplotypic associations defined by allelic DNA typing at HLA class I and class II loci in the Japanese population. Tissue Antigens. 2000; 56:522–529. PMID: 11169242.

12. Rossman MD, Thompson B, Frederick M, Maliarik M, Iannuzzi MC, Rybicki BA, et al. HLA-DRB1^*1101: a significant risk factor for sarcoidosis in blacks and whites. Am J Hum Genet. 2003; 73:720–735. PMID: 14508706.