Korean J Lab Med.  2007 Oct;27(5):360-368. 10.3343/kjlm.2007.27.5.360.

Evaluation of BioSewoom(TM) HLA-A, -B, -C PCR/SSP Kit

• Cha CH
• Kim MH
• Chung HJ
• Choi SE
• Oh HB

• KMID: 1781536
• DOI: http://doi.org/10.3343/kjlm.2007.27.5.360

Abstract

BACKGROUND: Human leukocyte antigen (HLA) typing based on polymerase chain reaction (PCR) is rapidly replacing the conventional serological method. This study was intended to evaluate Bio- SewoomTM HLA-A, -B, -C PCR/SSP kit (BioSewoom SSP) which had recently been developed in Korea. METHODS: A total of 158 samples from domestic (21) and international (137) HLA proficiency testing (PT) were genotyped with BioSewoom SSP, and its results were compared to consensus results. For comparison with INNO-LiPA HLA-A, -B, -C Typing Kit (INNO-LiPA, Innogenetics, Belgium), 20 samples of Koreans were genotyped with both kits for each HLA-A, -B, -C locus. RESULTS: Among the 21 samples of domestic PT, BioSewoom SSP showed ambiguities as follows: 4 samples (19.0%) in HLA-A, 6 (28.6%) in HLA-B, and 1 (4.8%) in HLA-C. The ambiguities could be resolved by considering the allele distribution of Koreans. Among the 137 samples from international PT, BioSewoom SSP also showed ambiguities as follows: 12 samples (8.8%) in HLA-A, 26 (19.0%) in HLA-B and 6 (4.4%) in HLA-C. Considering the allele distribution of Koreans, the serologic equivalents obtained from BioSewoom SSP showed a full agreement with those of INNO-LiPA in all the loci tested. Twelve (0.007%) among 1,760 PCR reactions from the 21 samples of domestic PT and 20 patient samples produced faint nonspecific bands, but it was negligible. PCR failure of internal control just barely occurred (15 PCR reactions, 0.009%), but it had no bearing on allele assignment. CONCLUSIONS: The performance of BioSewoom SSP was comparable to that of INNO-LiPA. All the ambiguities could be resolved by considering the allele distribution of Koreans. It is concluded that BioSewoom SSP has good performance to be used in routine HLA laboratories.

Keyword

HLA; SSP; DNA typing

MeSH Terms

Alleles
Genotype
HLA-A Antigens/*genetics
HLA-B Antigens/*genetics
HLA-C Antigens/*genetics
Histocompatibility Testing/*methods
Humans
Polymerase Chain Reaction/*methods
Reagent Kits, Diagnostic

Figure

• 

  Fig. 1. Examples of BioSewoom SSP band pattern. HLA-A: positive on A6, A7 and A13 lanes; HLA-B: positive on B2, B12, B15, B22, B23, B33, B39 and B47 lanes; HLA-C: positive on C4, C12 and C16 lanes.

• 

  Fig. 2. Analysis window of BioSewoom™ HLA SSP INT software. Input of positive band information (A2, A13) generated output of perfect matching genotype combination (A∗02-1/A∗33-1).

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