Korean J Lab Med.  2008 Jun;28(3):239-243. 10.3343/kjlm.2008.28.3.239.

Utility of In-House PCR for HLA-B27 Typing: Comparison of Concordance Rate between PCR Kit and In-House PCR

• Cho SY
• Lee KG
• Park SY
• Lee HJ

• KMID: 854873
• DOI: http://doi.org/10.3343/kjlm.2008.28.3.239

Abstract

BACKGROUND
Commercial kits of PCR method are widely used in HLA-B27 typing; however, their cost is relatively high. In this study, we evaluated the utility of an in-house PCR method by comparing it with that of a commercial kit.
METHODS
HLA-B27 typing was done in 188 patients by using two PCR methods, Absolute(TM) HLAB27 PCR kit (Biosewoom, Korea) and an in-house PCR method. The primers used in the in-house method were prepared by Bioneer (Korea). Both PCR tests were done by Gene Amp PCR System 9600 (Perkin-Elmer Centus Corp., USA).
RESULTS
The commercial kit and in-house PCR showed 100% concordance rate with each other in HLA-B27 typing. Of 188 patients tested 72 (38.3%) were positive and 116 (61.7%) were negative by the both tests. Of 62 patients with ankylosing spondylitis, 50 were positive (80.7%).
CONCLUSIONS
The in-house PCR is a reliable and cost-effective method and can replace or supplement commercial kits for HLA-B27 typing.

Keyword

HLA-B27; In-house PCR; DNA typing

MeSH Terms

Adult
Female
HLA-B27 Antigen/blood/*genetics
Histocompatibility Testing/*methods
Humans
Male
Polymerase Chain Reaction/*methods
Reagent Kits, Diagnostic
Sensitivity and Specificity

Figure

• 

  Fig. 1. Results of HLA-B27 typing. (A) Absolute™ HLA-B27 PCR kit (Biosewoom), (B) In-house PCR with B27 280 primer.

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