Lab Anim Res.  2016 Dec;32(4):241-248. 10.5625/lar.2016.32.4.241.

Changes of hematological references depends on storage period and temperature conditions in rats and dogs

• Lee JM
• Kang JS

• KMID: 2362916
• DOI: http://doi.org/10.5625/lar.2016.32.4.241

Abstract

Because changes in rat and dog hematological parameters according to storage conditions have been poorly documented, we sought to examine such changes. Blood analysis was performed using two hematology analyzers (ADVIA 2120i and Sysmex XN-V) after storage at room temperature and in cold storage for 5, 24, and 48 h, respectively. Interassay coefficients of variation for hematological parameters analyzed with the ADVIA 2120i and the XN-V showed similar. The levels of hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and platelet (PLT) showed significant variations with time in blood samples of rats and dogs. The leukocyte subpopulation showed high variation with storage conditions. The data for leukocyte differential counts obtained using the ADVIA 2120i, XN-V, and a manual differential counting procedure showed good agreement for neutrophils and lymphocyte counts, but monocytes, eosinophils, and basophils showed differences between the procedures. In conclusions, most rat and dog hematological parameters showed minimal changes; however, some showed high variation with storage time and temperature, especially PLT and leukocyte subpopulations. In conclusion, when performing hematological analysis in dogs and rats, it will be exactitude to analyze blood samples in fresh condition and at least within 24 h in the cold storage.

Keyword

Rat; dog; hematology; analysis; stability

MeSH Terms

Animals
Basophils
Blood Platelets
Dogs*
Eosinophils
Erythrocyte Indices
Hematocrit
Hematology
Leukocytes
Lymphocyte Count
Monocytes
Neutrophils
Rats*

Reference

1. Bourgès-Abella NH, Geffré A, Deshuillers PL, Braun JP, Trumel C. Changes in hematology measurements in healthy and diseased dog blood stored at room temperature for 24 and 48 hours using the XT-2000iV analyzer. Vet Clin Pathol. 2014; 43(1):24–35. PMID: 24512649.

2. Gulati GL, Hyland LJ, Kocher W, Schwarting R. Changes in automated complete blood cell count and differential leukocyte count results induced by storage of blood at room temperature. Arch Pathol Lab Med. 2002; 126(3):336–342. PMID: 11860310.

3. Goyal VK, Kakade S, Pandey SK, Gothi AK, Nirogi R. Determining the effect of storage conditions on prothrombin time, activated partial thromboplastin time and fibrinogen concentration in rat plasma samples. Lab Anim. 2015; 49(4):311–318. PMID: 26206586.

4. Médaille C, Briend-Marchal A, Braun JP. Stability of selected hematology variables in canine blood kept at room temperature in EDTA for 24 and 48 hours. Vet Clin Pathol. 2006; 35(1):18–23. PMID: 16511786.

5. Matsuzawa T. Present status of animal clinical pathology examinations in the Japanese Pharmaceutical Manufacturers Association. Toxicol Pathol. 1992; 20(3 Pt 2):528–533. PMID: 1296286.

6. Wood BL, Andrews J, Miller S, Sabath DE. Refrigerated storage improves the stability of the complete blood cell count and automated differential. Am J Clin Pathol. 1999; 112(5):687–695. PMID: 10549256.

7. Zandecki M, Genevieve F, Gerard J, Godon A. Spurious counts and spurious results on haematology analysers: a review. Part II: white blood cells, red blood cells, haemoglobin, red cell indices and reticulocytes. Int J Lab Hematol. 2007; 29(1):21–41. PMID: 17224005.

8. Bauer N, Nakagawa J, Dunker C, Failing K, Moritz A. Evaluation of the automated hematology analyzer Sysmex XT-2000iV ™ compared to the ADVIA ® 2120 for its use in dogs, cats, and horses. Part II: Accuracy of leukocyte differential and reticulocyte count, impact of anticoagulant and sample aging. J Vet Diagn Invest. 2012; 24(1):74–89. PMID: 22362937.

9. England JM, Rowan RM, van Assendelft OW, Bull BS, Coulter W, Fujimoto K, Groner W, Richardson-Jones A, Klee G, Koepke JA, Lewis SM, McLaren CE, Shinton NK, Tatsumi N, Verwilghen RL. Recommendations of the International Council for Standardization in Haematology for Ethylenediaminetetraacetic Acid Anticoagulation of Blood for Blood Cell Counting and Sizing. International Council for Standardization in Haematology: Expert Panel on Cytometry. Am J Clin Pathol. 1993; 100(4):371–372. PMID: 8213631.

10. Schoorl M, Schoorl M, Oomes J, van Pelt J. New fluorescent method (PLT-F) on Sysmex XN2000 hematology analyzer achieved higher accuracy in low platelet counting. Am J Clin Pathol. 2013; 140(4):495–499. PMID: 24045545.

11. Wada A, Takagi Y, Kono M, Morikawa T. Accuracy of a New Platelet Count System (PLT-F) Depends on the Staining Property of Its Reagents. PLoS One. 2015; 10(10):e0141311. PMID: 26496387.

12. Meintker L, Ringwald J, Rauh M, Krause SW. Comparison of automated differential blood cell counts from Abbott Sapphire, Siemens Advia 120, Beckman Coulter DxH 800, and Sysmex XE-2100 in normal and pathologic samples. Am J Clin Pathol. 2013; 139(5):641–650. PMID: 23596116.

13. Tan BT, Nava AJ, George TI. Evaluation of the Beckman Coulter UniCel DxH 800, Beckman Coulter LH 780, and Abbott Diagnostics Cell-Dyn Sapphire hematology analyzers on adult specimens in a tertiary care hospital. Am J Clin Pathol. 2011; 135(6):939–951. PMID: 21571967.

14. Zandecki M, Genevieve F, Gerard J, Godon A. Spurious counts and spurious results on haematology analysers: a review. Part I: platelets. Int J Lab Hematol. 2007; 29(1):4–20. PMID: 17224004.