Ann Dermatol.  1991 Jul;3(2):91-95. 10.5021/ad.1991.3.2.91.

Easy Application of Digoxigenin-11-dUTP Labelled Probe in Detection of Human Papillomavirus DNA

• Park KJ
• Sung HS

• KMID: 2361999
• DOI: http://doi.org/10.5021/ad.1991.3.2.91

Abstract

In situ hybridization was performed in ten cases of condyloma acuminata in order to study the applicability of digoxigenin-11 dUTP(Dig-dUTP) labelled probe compared with radioactive isotope labelled probes. Although signal intensity was denser in radiolabelled probes, high positive rates were obtained with Dig-dUTP labelled probes. From these results, Dig-dUTP labelling is found to be more efficient in typing of human papillomavirus DNA than radiolabelling.

Keyword

In situ hybridization; Condyloma acuminatum; Digoxigenin-11-dUTP; Radioactive isotope

MeSH Terms

DNA*
Humans*
In Situ Hybridization
DNA