J Vet Sci.  2004 Mar;5(1):71-73.

Optimization of in situ hybridization assay using non-radioactive DNA probes for the detection of canine herpesvirus (CHV) in paraffin-embedded sections

• Kim O

Abstract

Two non-radioactive probes using digoxigenin or biotin were developed for detecting canine herpesvirus (CHV) and compared for their sensitivities by in situ hybridization (ISH) in formalin fixed, paraffin embedded sections, which has been used routinely in veterinary fields. Sections of the CHV-infected cell preparation were subjected to several different ISH protocols using digoxigenin- or biotin-labeled probe respectively. Results were compared for the hybridization and background signal intensities. The best result was obtained by the optimized ISH protocol using digoxigenin-labeled probe for detection of CHV DNA. The optimized ISH assay, which developed in this study, may be a valid tool for the study of pathogenesis and diagnosis of CHV infection.

Keyword

canine herpesvirus; digoxigenin; biotin; in situ hybridization

MeSH Terms

Animals
Biotin/diagnostic use
Cell Line
DNA Probes/chemistry/genetics
DNA, Viral/chemistry/genetics
Digoxigenin/diagnostic use
Dog Diseases/diagnosis/*virology
Dogs
Herpesviridae Infections/diagnosis/*veterinary/virology
Herpesvirus 1, Canid/genetics/*isolation&purification
In Situ Hybridization/methods/*veterinary
Polymerase Chain Reaction/veterinary
Sensitivity and Specificity