Tissue Eng Regen Med.
2016 Feb;13(1):13-20. 10.1007/s13770-015-0029-z.
Optimization of Recombinant Human Platelet-Derived Growth Factor-BB Encapsulated in Poly (Lactic-co-Glycolic Acid) Microspheres for Applications in Wound Healing
- Affiliations
-
- 1Department of Biomedical Science, Graduate School, Kyungpook National University, Daegu, Korea. jolim@knu.ac.kr
- 2Korea Institute of Industrial Technology, Biomedical Manufacturing Technology Center, Daegu, Korea.
- 3Department of Food Science and Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University, Yongin, Korea.
- 4Department of Materials Science and Metallurgy, College of Engineering, Kyungpook National University, Daegu, Korea.
- 5Biomedical Research Institute, Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu, Korea.
- KMID: 2360862
- DOI: http://doi.org/10.1007/s13770-015-0029-z
Abstract
- Growth factors play multiple and critical roles in wound repair processes. Platelet-derived growth factor (PDGF) is a potent growth factor that is particularly important in the early inflammatory phase of wound healing. In order to extend the half-life of PDGF, polymeric encapsulation is used. In the current study, Poly (lactic-co-glycolic acid) (PLGA) microspheres containing recombinant human (rh) PDGF-BB were prepared to prolong the effectiveness of this growth factor. PLGA microspheres were optimized using a modified w/o/w-double-emulsion/solvent evaporation method by changing the processing conditions of stirring speed and emulsifier (polyvinyl alcohol) concentration. Microspheres prepared using the optimized method released rhPDGF-BB for up to three weeks. An in vitro migration assay showed a significant decrease in the wound area in cells treated with rhPDGF-BB microspheres compared to control cells. These findings demonstrate the potential of rhPDGF-BB encapsulated in microspheres to enhance wound healing.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Biodegradable poly (lactic acid) microspheres for drug delivery systems
- Level of Platelet Derived Growth Factor(PDGF) in Blood Bank Platelet Concentrate
- PLGA Microspheres Containing Hydrophobically Modified Magnesium Hydroxide Particles for Acid NeutralizationMediated Anti-Inflammation
- The Effect of PDGF-Loaded Biodegradable Membrane on Early Healing Stage in Guided Tissue Regeneration
- Leukocyte-Poor Platelet-Rich Plasma-Derived Growth Factors Enhance Human Fibroblast Proliferation In Vitro
