J Korean Soc Plast Reconstr Surg.  2006 Nov;33(6):732-736.

Level of Platelet Derived Growth Factor(PDGF) in Blood Bank Platelet Concentrate

• Hong YT
• Han SK
• Lee BI
• Kim WK

Abstract

PURPOSE: The purpose of this pilot study was to investigate a potential of platelet concentrate obtained from blood bank(PCBB) in accelerating wound healing and to determine an effective treatment protocol by quantifying levels of platelet derived growth factor (PDGF)-BB in PCBB in vitro.
METHODS
The first study was designed to investigate quantity of PDGF-BB over stored time of the PCBB. The stored times for each PCBB were 1, 3, 5, 7, 9, 11 and 13 days. The second study was designed to determine efficacy of adding thrombin to stimulate release of PDGF-BB from the platelets of PCBB. The platelets were suspended and incubated in either with or without thrombin. On 30 minutes and days 1, 3, 5, 7 after incubation, the levels of PDGF-BB were measured.
RESULTS
PDGF-BB level showed a linear decrease over stored time of PCBB from the first day to the 13th day. Addition of thrombin increased PDGF-BB release from 30 minute through the 5th day.
CONCLUSION
The results indicate that PCBB can provide sufficient amount of growth factors to stimulate wound healing and adding thrombin accelerate it.

Keyword

Platelet; Wound healing; Blood bank

MeSH Terms

Blood Banks*
Blood Platelets*
Clinical Protocols
Intercellular Signaling Peptides and Proteins
Pilot Projects
Platelet-Derived Growth Factor
Thrombin
Wound Healing
Intercellular Signaling Peptides and Proteins
Platelet-Derived Growth Factor
Thrombin