J Korean Med Sci.  2015 Dec;30(12):1754-1763. 10.3346/jkms.2015.30.12.1754.

Human Urine-derived Stem Cells Seeded Surface Modified Composite Scaffold Grafts for Bladder Reconstruction in a Rat Model

Affiliations
  • 1Department of Urology, Kyungpook National University School of Medicine, Daegu, Korea. tgkwon@knu.ac.kr
  • 2Bio-Medical Research Institute, Kyungpook National University Hospital, Daegu, Korea.
  • 3Department of Neural Development and Disease, Korea Brain Research Institute, Daegu, Korea.
  • 4Department of Laboratory Animal Research Support Team, Yeungnam University, Daegu, Korea.
  • 5Department of Nanobiomedical Science & WCU Research Center, Dankook University, Cheonan, Korea.
  • 6Department of Urology, Yeungnam University College of Medicine, Daegu, Korea.
  • 7Department of Advanced Materials, Hannam University, Daejeon, Korea.
  • 8Department of Emergency Medicine, Kyungpook National University School of Medicine, Daegu, Korea.

Abstract

We conducted this study to investigate the synergistic effect of human urine-derived stem cells (USCs) and surface modified composite scaffold for bladder reconstruction in a rat model. The composite scaffold (Polycaprolactone/Pluronic F127/3 wt% bladder submucosa matrix) was fabricated using an immersion precipitation method, and heparin was immobilized on the surface via covalent conjugation. Basic fibroblast growth factor (bFGF) was loaded onto the heparin-immobilized scaffold by a simple dipping method. In maximal bladder capacity and compliance analysis at 8 weeks post operation, the USCs-scaffold(heparin-bFGF) group showed significant functional improvement (2.34 ± 0.25 mL and 55.09 ± 11.81 microL/cm H2O) compared to the other groups (2.60 ± 0.23 mL and 56.14 ± 9.00 microL/cm H2O for the control group, 1.46 ± 0.18 mL and 34.27 ± 4.42 microL/cm H2O for the partial cystectomy group, 1.76 ± 0.22 mL and 35.62 ± 6.69 microL/cm H2O for the scaffold group, and 1.92 ± 0.29 mL and 40.74 ± 7.88 microL/cm H2O for the scaffold(heparin-bFGF) group, respectively). In histological and immunohistochemical analysis, the USC-scaffold(heparin-bFGF) group showed pronounced, well-differentiated, and organized smooth muscle bundle formation, a multi-layered and pan-cytokeratin-positive urothelium, and high condensation of submucosal area. The USCs seeded scaffold(heparin-bFGF) exhibits significantly increased bladder capacity, compliance, regeneration of smooth muscle tissue, multi-layered urothelium, and condensed submucosa layers at the in vivo study.

Keyword

Bladder Regeneration; Surface Modified Scaffold; Urine-derived Stem Cells; Basic Fibroblast Growth Factor 2

MeSH Terms

Adult Stem Cells/cytology/metabolism/*transplantation
Animals
Biocompatible Materials/chemistry
Cell Differentiation
Fibroblast Growth Factor 2/administration & dosage
Heparin/administration & dosage
Humans
Materials Testing
Models, Animal
Poloxamer
Polyesters
Rats
Reconstructive Surgical Procedures
Regeneration
Tissue Engineering/*methods
Tissue Scaffolds/chemistry
Urinary Bladder/anatomy & histology/physiology/*surgery
Urine/*cytology
Biocompatible Materials
Fibroblast Growth Factor 2
Heparin
Poloxamer
Polyesters
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