Ann Dermatol.
1999 Jul;11(3):135-141. 10.5021/ad.1999.11.3.135.
Identification of Trichophyton tonsurans by Random Amplified Polymorphic DNA
- KMID: 2359717
- DOI: http://doi.org/10.5021/ad.1999.11.3.135
Abstract
- BACKGROUND
T. tonsurans is an anthropophilic dermatophyte mostly causing tinea capitis and tinea corporis. In East Asian countries, it has rarely been isolated until now. However, it is necessary for researchers in Asian countries to be more accustomed to T. tonsurans than before because of frequent international sports exchanges nowadays.
OBJECTIVES
This study was performed to identify T. tonsurans by random amplified polymorphic DNA (RAPD) analysis.
METHODS
Fifteen strains which were tentatively identified as T. tonsurans in Brazil were identified again by several conventional mycological tests and RAPD analysis.
RESULTS
Among 15 Brazilian strains, 3 were identified as T. tonsurans, 8 T. mentagrophytes, 3 T. nJmwn and 1 T. raubitschekii by conventional mycological tests. This result was examined again by RAPD analysis.
CONCLUSION
RAPD analysis is considered a rapid and reliable method for identification of T. tonsurans if the procedure is carefully standardized with adequate-primers.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Identification and phylogenetic relationship of dermatophytes based on RFLP analysis and nucleotide sequence of internal transcribed spacer (ITS)1 in nuclear ribosome DNA
- Trichophyton tonsurans Infection in Wrestlers and a Child
- Trichophyton tonsurans Infection in a Judoist Girl and Her Mother
- Identification and Subtyping of Trichophyton rubrum by Molecular Biological Methods
- A Case of Tinea Pseudoimbricata by Trichophyton tonsurans
