Ann Dermatol.  1999 Jul;11(3):135-141. 10.5021/ad.1999.11.3.135.

Identification of Trichophyton tonsurans by Random Amplified Polymorphic DNA

Abstract

BACKGROUND
T. tonsurans is an anthropophilic dermatophyte mostly causing tinea capitis and tinea corporis. In East Asian countries, it has rarely been isolated until now. However, it is necessary for researchers in Asian countries to be more accustomed to T. tonsurans than before because of frequent international sports exchanges nowadays.
OBJECTIVES
This study was performed to identify T. tonsurans by random amplified polymorphic DNA (RAPD) analysis.
METHODS
Fifteen strains which were tentatively identified as T. tonsurans in Brazil were identified again by several conventional mycological tests and RAPD analysis.
RESULTS
Among 15 Brazilian strains, 3 were identified as T. tonsurans, 8 T. mentagrophytes, 3 T. nJmwn and 1 T. raubitschekii by conventional mycological tests. This result was examined again by RAPD analysis.
CONCLUSION
RAPD analysis is considered a rapid and reliable method for identification of T. tonsurans if the procedure is carefully standardized with adequate-primers.

Keyword

Trichophyton tonsurans; Random amplified polymorphic DNA; Dermatophyte; Identification; Fungus

MeSH Terms

Arthrodermataceae
Asian Continental Ancestry Group
Brazil
DNA*
Fungi
Humans
Methods
Sports
Tinea
Tinea Capitis
Trichophyton*
DNA
Full Text Links
  • AD
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr