Restor Dent Endod.  2016 Aug;41(3):196-201. 10.5395/rde.2016.41.3.196.

Effect of three nanobiomaterials on microhardness of bleached enamel

• Khoroushi
• Shirban F
• Kaveh S
• Doustfateme S

• KMID: 2346768
• DOI: http://doi.org/10.5395/rde.2016.41.3.196

Abstract

OBJECTIVES
The aim of this in vitro study was to evaluate the effect of incorporating three different nanobiomaterials into bleaching material on microhardness of bleached enamel.
MATERIALS AND METHODS
The crowns of 24 extracted sound human molars were sectioned. Sixty enamel specimens (2 × 3 × 4 mm) were selected and divided into five groups (n = 12): Group 1 received no bleaching procedure (control); Group 2 underwent bleaching with a 40% hydrogen peroxide (HP) gel; Groups 3, 4, and 5 were bleached with a 40% HP gel modified by incorporation of bioactive glass (BAG), amorphous calcium phosphate (ACP) and hydroxyapatite (HA), respectively. The enamel microhardness was evaluated. The differences in Knoop microhardness data of each group were analyzed by one-way ANOVA, followed by post hoc Tukey tests.
RESULTS
Significant differences were observed between the study groups. The enamel microhardness changes in Groups 1, 3, 4, and 5 were significantly lower than that of Group 2 (p < 0.001).
CONCLUSIONS
Within the limitations of this study, it can be concluded that incorporation of each one of the three tested biomaterials as remineralizing agents might be effective in decreasing enamel microhardness changes subsequent to in-office bleaching.

Keyword

Amorphous calcium phosphate; Bioactive glass; Bleaching; Enamel; Hardness; Hydroxyapatite

MeSH Terms

Biocompatible Materials
Calcium
Crowns
Dental Enamel*
Durapatite
Glass
Hardness
Humans
Hydrogen Peroxide
In Vitro Techniques
Molar
Biocompatible Materials
Calcium
Durapatite
Hydrogen Peroxide

Figure

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  Figure 1 Atomic force microscopy (AFM) images. (a) Group 1, Intact enamel; (b) Group 2, Bleached enamel with a 40% hydrogen peroxide; (c) Bleached enamel with a 40% hydrogen peroxide gel modified by bioactive glass; (d) Bleached enamel with a 40% hydrogen peroxide gel modified by amorphous calcium phosphate; (e) Bleached enamel with a 40% hydrogen peroxide gel modified by hydroxyapatite. In Group 2, AFM evaluation exhibited irregular surface topography with more structure removal than in Group 1. In Group 4, the surface of specimens looked more prominent and dense than Group 1.

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