J Korean Med Sci.  2015 Sep;30(9):1313-1320. 10.3346/jkms.2015.30.9.1313.

Effects of Moderate Alcohol Intake in the Bladder of the Otsuka Long Evans Tokushima Fatty Diabetic Rats

• Bae WJ
• Choi YS
• Kim SJ
• Cho HJ
• Hong SH
• Kim SW
• Hwang TK
• Kim DJ
• Lee JY

• KMID: 2344158
• DOI: http://doi.org/10.3346/jkms.2015.30.9.1313

Abstract

Diabetes is related with a number of cystopathic complications. However, there have been no studies about the influence of alcohol consumption in the bladder of type 2 diabetes. Thus, we investigated the effect of moderate alcohol intake in the bladder of the Otsuka Long Evans Tokushima Fatty (OLETF) diabetic rat. The non-diabetic Long-Evans Tokushima Otsuka (LETO, n=14) and the OLETF control group (n=14) were fed an isocaloric diet; the LETO (n=14) and the OLETF ethanol group (n=14) were fed 36% ethanol 7 g/kg/day. After ten weeks, muscarinic receptors, RhoGEFs, myogenic change, and the level of oxidative stress were evaluated. Moderate alcohol intake significantly decreased excessive muscarinic receptor and Rho kinase expressions in the OLETF rats compared with the LETO rats. In addition, iNOS and collagen expression were not changed in the OLETF rats in spite of alcohol consumption. Superoxide dismutase levels, which is involved in antioxidant defense, in the LETO rats were significantly decreased after alcohol consumption, however those in the OLETF rats were similar. Moderate alcohol consumption reduces the oxidative stress, and may prevent molecular and pathologic changes of the bladder of rats with type 2 diabetes.

Keyword

Alcohols; Diabetes Mellitus; Urinary Bladder Disease

MeSH Terms

Alcohol Drinking/adverse effects
Animals
Diabetes Mellitus, Type 2/*complications/*metabolism/pathology
Ethanol/*toxicity
Humans
Rats
Rats, Inbred OLETF
Reactive Oxygen Species/*metabolism
Urinary Bladder/*drug effects/*metabolism/pathology
Reactive Oxygen Species
Ethanol

Figure

• 

  Fig. 1 Comparison for bladder M2-mAChR, M3-mAChR mRNA expressions among 4 groups. (A) RT-PCR analysis (B) Densitometric analysis relative to beta-actin of M2-mAChR (C) Densitometric analysis relative to beta-actin of M3-mAChR. *P < 0.05. LC, LETO-Control; LE, LETO-Ethanol; OC, OLETF-Control; OE, OLETF-Ethanol.

• 

  Fig. 2 Comparison for bladder RhoGEFs mRNA expressions (A) and RhoA, ROCK-I, ROCK-II protein expressions (B) among 4 groups. *P < 0.05. LC, LETO-Control; LE, LETO-Ethanol; OC, OLETF-Control; OE, OLETF-Ethanol.

• 

  Fig. 3 Comparison of total superoxide dismutase expression. *P < 0.05. LC, LETO-Control; LE, LETO-Ethanol; OC, OLETF-Control; OE, OLETF-Ethanol.

• 

  Fig. 4 Comparison for histology among 4 groups (OE, OC, LE, and LC). (A) Masson's trichrome staining. (B) Bladder smooth muscle/collagen ratio. Scale bars shown in each figure represent 100 µm. *P < 0.05. LC, LETO-Control; LE, LETO-Ethanol; OC, OLETF-Control; OE, OLETF-Ethanol.

• 

  Fig. 5 Comparison for immunohistochemistry among 4 groups (OE, OC, LE, and LC). (A) Immunohistochemical staining of iNOS. (B) Mean number of iNOS-positive cells. Scale bars shown in each figure represent 100 µm. *P < 0.05. LC, LETO-Control; LE, LETO-Ethanol; OC, OLETF-Control; OE, OLETF-Ethanol.

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