The Instillation Interval of 5% NaCl Eye Drops to Treat Corneal Edema in Rabbit

Chun, Yeoun Sook; Kim, Jeong Hun; Park, In Ki; Wee, Won Ryang; Lee, Jin Hak

Abstract

The purpose of this study was to investigate the maximum effect and effective instillation time interval of 5% NaCl on the corneal edema. Onetenth ml of 2% lidocaine was injected intracamerally to make the corneal edema at right eyes of four rabbits, but any manipulation was not done on the left eyes. Twelve hours after the operation, one drop of 5% NaCl was instillated in both eyes and the central corneal thickness(CCT)was measured every 30 minutes until the three successive records had no demonstrable changes compared to baseline levels. Maximum effect on normal cornea and corneal edema showed at 60 minutes after the instillation of one drop of 5% NaCl;the CCT decreased by about 5% compared to preopertaive level. To evaluate the effective instillation time interval, twenty eyes of 20 white rabbits were randomly divided into 5 groups and 0.1 ml of 2% lidocaine was injected intracamerally at right eyes. In control group, they were remained without any medications and showed natural course of corneal edema. 7In group 1, they were treated with 5% NaCl every 30 minutes and in group 2, 60 minutes, in group 3, 120 minutes, and in group 4, 180 minutes. Using a ultrasonic pachymeter, CCT was measured at 30 minutes interval up to 360 minutes. In group 1, decrease of the CCT was found to be more than 5% at 60 minutes after treatment and there was 5.00~7.24% decrease of CCT up to 360 minutes. In group 2 and 3, decrease of the CCT more than 5% was found at 90 minutes after treatment and there was 3.95~6.07% decrease of CCT up to 360 minutes.In group 4, decrease of the CCT more than 5% was found at 90 minutes after treatment and there was severe fluctuation of the decrease of the CCT from 2.47% to 6.78% up to 360 minutes. In group 1, decrease of the CCT was larger than that of the others, but there was chances of the corneal epithelial toxicity and incovenience due to frequent instillation. In group 2 and 3, there was no difference between the two groups in the amounts of the decrease of the CCT, but in terms of convenience, instillation every 2 hours(120 minutes)was recommendable. Treatment with 5% NaCl every 2 hours on corneal stromal edema with normal epithelial barrier function can decrease the CCT and induce good maintenance effect.