J Korean Ophthalmol Soc.  2000 Mar;41(3):543-549.

The Study of Toxicity of Mitomycin C and Steroid as Eyedrops in Rabbit Cornea

• Lee HI
• Yi K
• Moon NJ

Abstract

To investigate the drug effect of topical Mitomycin C in various concentrations combined with steroid, we used 30 rabbits whose corneal epitheliums were abraded intentionally. They were divided into 5 groups. Using MMC in various concentrations and steroids, we instilled them into right eyes and saline into left eyes as control. We measured the healing rate at a interval of 12 hours. Three months later, we sacrificed one of them and examined the pathological changes from various ocular tissues. We found many complications such as corneal edema, ulcer, opacity and definite delay of wound healing when the concentration higher than 0.04%MMC was used. There were also decreased fibroblast cells in H-E staining. The steroids seemed not to pose any influence on the effect of MMC. In conclusion, we must take the great care in the use of MMC, and the concentration should be low when used. In addition, we still think there ought to be more studies as to the effect of steroids according to MMC levels.

Keyword

Corneal epithelium; Mitomycin C; Rabbit; Steroids

MeSH Terms

Cornea*
Corneal Edema
Epithelium, Corneal
Fibroblasts
Intention
Mitomycin*
Ophthalmic Solutions*
Rabbits
Steroids
Ulcer
Wound Healing
Mitomycin
Ophthalmic Solutions
Steroids