J Korean Pain Soc.
2001 Dec;14(2):136-141.
Neurotoxicity of Sodium Salicylate on Spinal Sensory Neurons in Culture
- Affiliations
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- 1Department of Anesthesiology & Pain Clinic, School of Medicine, Wonkwang University, Iksan, Korea.
- 2Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Korea.
Abstract
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BACKGROUND: Sodium salicylate (SS) is a nonsteroidal anti-inflammatory drug (NSAID) for the treatment of neuralgia or pain from rheumatoid arthritis. When abused or used in excess, SS can induce cytotoxicity. The present study examined whether SS has a neurotoxic effect.
METHODS
Cell viability was examined by MTT [3-(4,5-dimethylthiazol-2,5-dipheny ltetrazolium bromide] assay and Sulforhodamine (SRB) assay after cultivating dorsal root ganglion (DRG) neurons derived from neonatal mouse. These cells were treated with various concentrations of SS for 24 hours. In addition, the amount of protein synthesis against SS was measured in these cultures.
RESULTS
Cell viability (20, 40ng/ml SS) significantly decreased in a dose-dependent manner. Additionally, SS inhibited protein synthesis after the exposure of cultured mouse DRG neurons to 30 ng/ml of SS for 24 hours.
CONCLUSIONS
The present study suggests that SS is toxic in cultured DRG neurons derived from neonatal mouse by decreasing cell viability and the amount of protein synthesis.
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