Exp Mol Med.
2016 Jan;48(1):e203. 10.1038/emm.2015.97.
Applications of stable, nonradioactive isotope tracers in in vivo human metabolic research
- Affiliations
-
- 1Department of Geriatrics, the Center for Translational Research on Aging and Longevity, Donald W. Reynolds Institute on Aging, College of Medicine, The University of Arkansas for Medical Sciences, Little Rock, AR, USA. iykim@uams.edu
- 2Deparatment of Physical Education, College of Sciences in Education, Yonsei University, Seoul, Republic of Korea.
- 3Section of Endocrinology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
- KMID: 2327676
- DOI: http://doi.org/10.1038/emm.2015.97
Abstract
- The human body is in a constant state of turnover, that is, being synthesized, broken down and/or converted to different compounds. The dynamic nature of in vivo kinetics of human metabolism at rest and in stressed conditions such as exercise and pathophysiological conditions such as diabetes and cancer can be quantitatively assessed with stable, nonradioactive isotope tracers in conjunction with gas or liquid chromatography mass spectrometry and modeling. Although measurements of metabolite concentrations have been useful as general indicators of one's health status, critical information on in vivo kinetics of metabolites such as rates of production, appearance or disappearance of metabolites are not provided. Over the past decades, stable, nonradioactive isotope tracers have been used to provide information on dynamics of specific metabolites. Stable isotope tracers can be used in conjunction with molecular and cellular biology tools, thereby providing an in-depth dynamic assessment of metabolic changes, as well as simultaneous investigation of the molecular basis for the observed kinetic responses. In this review, we will introduce basic principles of stable isotope methodology for tracing in vivo kinetics of human or animal metabolism with examples of quantifying certain aspects of in vivo kinetics of carbohydrate, lipid and protein metabolism.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Exploring Human Muscle Dynamics In Vivo Using Stable Isotope Tracers
- In Vivo and In Vitro Quantification of Glucose Kinetics: From Bedside to Bench
- The association between carbon and nitrogen stable isotope ratios of human hair and hypertension
- Stable isotope analysis of Joseon people skeletons from the cemeteries of Old Seoul City, the capital of Joseon Dynasty
- Arterial Spin Labelling-Based Blood-Brain Barrier Assessment and Its Applications
