J Korean Acad Periodontol.  2008 Sep;38(3):543-550.

Pro-inflammatory cytokine expression in human gingival fibroblasts by Tannerella forsythia whole bacteria, membrane proteins, and lipopolysaccharide

Affiliations
  • 1Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, Korea. icrhyu@snu.ac.kr
  • 2Oral Infection and Immunity, School of Dentistry, Seoul National University, Korea.

Abstract

PURPOSE
The purpose of this study was to investigate induction of cytokine expression in human gingival fibroblasts (HGFs) by whole cell and the components of T. forsythia. MATERIAL AND METHODS: After HGFs were treated with lipopolysaccharide (LPS), membrane protein isolated from T. forsythia or culture media of T. forsythia, the induction of interleukin (IL)-1, IL-6 and IL-8 was examined with real-time PCR and ELISA. Their induction ability of cytokines was compared with whole bacteria. RESULT: The expression of IL-6 and IL-8 was significantly induced in HGFs by whole bacteria and membrane protein. The expression of IL-1beta was induced by membrane protein of T. forsythia, not by whole bacteria. LPS and condition media of T. forsythia slightly activated HGFs.
CONCLUSION
The membrane protein of T. forsythia could be one of virulence factors.

Keyword

Tannerella forsythia; virulence factors; human gingival fibroblasts

MeSH Terms

Bacteria
Culture Media
Cytokines
Enzyme-Linked Immunosorbent Assay
Fibroblasts
Forsythia
Humans
Interleukin-6
Interleukin-8
Interleukins
Membrane Proteins
Membranes
Real-Time Polymerase Chain Reaction
Virulence Factors
Culture Media
Cytokines
Interleukin-6
Interleukin-8
Interleukins
Membrane Proteins
Virulence Factors
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