Yeungnam Univ J Med.  1993 Dec;10(2):360-368.

Isolation of GTP binding from bovine brain

Abstract

GTP binding protein (G-protein) associated with membrane and involved in signal transduction was isolated from bovine brain, and molecular weight of G protein was observed. As the results, cell membranes were homogenized from bovine brain tissues and proteins of membrane were gained using 1% cholate, and progressed the chromatography. The purification process was performed by step, DEAE-Sephacel, Ulttrogel AcA 34 and heptylamine-Sepharose column chromatography. The chromatographic fractions were confirmed by GTP binding assay and SDS-polyacrylamide gel electrophoresis. Molecular weight of Goa was revealed 39,000 dalton and GR 36,000 dalton. One more step of heptylamine-Sepharose was enforced to purify the GTP binding protein. Finally I gained the GTP binding protein isolated subtype of Goalpha and Gbeta.


MeSH Terms

Brain*
Cell Membrane
Cholates
Chromatography
Electrophoresis
GTP-Binding Proteins
Guanosine Triphosphate*
Membranes
Molecular Weight
Signal Transduction
Cholates
GTP-Binding Proteins
Guanosine Triphosphate
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