Tuberc Respir Dis.  2010 Nov;69(5):331-336.

Five Rare Non-Tuberculous Mycobacteria Species Isolated from Clinical Specimens

• Park YK
• Lee YJ
• Yu H
• Jeong MY
• Ryoo SW
• Kim CK
• Kim HJ

Abstract

BACKGROUND
Recently, the rate of infections with non-tuberculous mycobacteria (NTM) has been increasing in Korea. Precise identification of NTM is critical to determination of the pathogen and to target treatment of NTM patients.
METHODS
Sixty-eight unclassified mycobacteria isolates by rpoB PCR-RFLP assay (PRA) collected in 2008 were analyzed by National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST) search after sequencing of 16S rRNA, hsp65, rpoB genes.
RESULTS
Nineteen strains of 68 isolates were specified as species after sequencing analysis of 3 gene types. We found 3 M. lentifulavum, 5 M. arupense, 4 M. triviale, 4 M. parascrofulaceum, and one M. obuense. One M. tuberculosis and another M. peregrinum were mutated at the Msp I recognition site needed for rpoB PRA. The remaining 49 isolates did not coincide with identical species at the 3 kinds genes.
CONCLUSION
Sequencing analysis of 16S rRNA, hsp65, rpoB was useful for identification of NTM unclassified by rpoB PRA.

Keyword

Mycobacteria, Atypical; RNA, Ribosomal, 16S; rpoB protein; Heat-Shock Protein 64

MeSH Terms

Biotechnology
Korea
Nontuberculous Mycobacteria
RNA, Ribosomal, 16S
Tuberculosis
RNA, Ribosomal, 16S

Figure

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  Figure 1 Comparison of variation region of 16S rRNA according to species. The number is nucleotide order of 16S rRNA gene of M. tuberculosis H37Rv.

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  Figure 2 Comparison of variation region of hsp65 gene according to species. The number is nucleotide order of hsp65 gene of M. tuberculosis H37Rv.

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  Figure 3 Comparison of variation region of rpoB gene according to species. The number is nucleotide order of rpoB gene of M. tuberculosis H37Rv.

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