Tuberc Respir Dis.  2006 Jan;60(1):83-91.

Effects of Aspirin on the Pathogenesis of Acute Lung Injury in Rats Subjected to Hemorrhage

• Park YY
• Lee YM

Abstract

BACKGROUND: For unknown reasons, the serum ferritin concentrations are higher in patients with acute lung injury. A pretreatment with aspirin reduces the acute lung injury in rats subjected severe hemorrhage, and increases the rate of ferritin synthesis in vitro. This study investigated the effect of aspirin on the serum ferritin changes in rats subjected to severe hemorrhage.
METHODS
Hemorrhagic shock was induced by withdrawing blood (20 ml/kg of B.W.) through the femoral artery for 5 min. The rats were pretreated with aspirin (10 mg/kg, i.v.) 30 min before hemorrhage.
RESULTS
The protein content and leukocyte count in the bronchoalveolar lavage fluid, lung tissue myeloperoxidase activities were significantly higher after hemorrhage. The aspirin pretreatment prevented these changes. The serum and lavage fluid ferritin concentrations were elevated higher after hemorrhage. These were also attenuated by the aspirin pretreatment.
CONCLUSION
The changes in the serum and lung lavage ferritin level might be closely related to the severity of hemorrhage?induced acute lung injury. Therefore, the serum and lavage ferritin concentrations can be a useful biomarker for patients with precipitating conditions.

Keyword

Hemorrhage; Acute lung injury; Aspirin; Ferritin

MeSH Terms

Acute Lung Injury*
Animals
Aspirin*
Bronchoalveolar Lavage
Bronchoalveolar Lavage Fluid
Femoral Artery
Ferritins
Hemorrhage*
Humans
Leukocyte Count
Lung
Peroxidase
Rats*
Shock, Hemorrhagic
Therapeutic Irrigation
Aspirin
Ferritins
Peroxidase

Figure

• 

  Figure 1 Changes in mean arterial pressure following hemorrhage. Each point is the mean±SEM for the number of determinations shown in parentheses.

• 

  Figure 2 Two hours after hemorhage, the hemorrhaged rats had increased bronchoalveolar lavage leukocyte numbers compared to sham-treated rats. Pretreatment with mepacrine (Mepa) or aspirin (ASA) decreased the leukocyte counts. The data shown is the mean±SEM for the number of determinations shown in parentheses. **p<0.01, ***p<0.001 compared to Sham. ##p<0.01, ###p<0.001 compared to Hemo.

• 

  Figure 3 Rats subjected to hemorrhage had increased lung tissue myeloperoxidase (MPO) activities compared to Sham rats. These changes were attenuated by pretreatment with mepacrine (Mepa) or aspirin (ASA). The data shown is the mean±SEM for the number of determinations shown in parentheses. *p<0.05, **p<0.01, ***p<0.001 compared to Sham. #p<0.05, ##p<0.01 compared to Hemo.

• 

  Figure 4 Two hours after hemorhage, the hemorrhaged rats had increased bronchoalveolar lavage protein concentrations compared to sham-treated rats. These changes were prevented by pretreatment with mepacrine (Mepa) or aspirin (ASA). The data shown is the mean±SEM for the number of determinations shown in parentheses. ***p<0.001 compared to Sham. ###p<0.001 compared to Hemo.

• 

  Figure 5 Rats subjected to hemorrhage had increased bronchoalveolar lavage ferritin concentrations compared to sham-treated rats. Pretreatment with mepacrine (Mepa) or aspirin (ASA) decreased the increase of lavage ferritin concentrations. The data shown is the mean±SEM for the number of determinations shown in parentheses. *p<0.05 compared to Sham. #p<0.05, ##p<0.01 compared to Hemo.

• 

  Figure 6 The serum ferritin concentrations of Sham rats did not change through the 2-hour period following hemorrhage. In contrast, the serum ferritin concentrations of hemorrhaged rats increased progressively following hemorrhage. By comparison, the serum ferritin concentrations of mepacrine or aspirin-treated hemorrhaged rats were decreased compared to the values of hemorrhaged rats without pretreatment. Each point is the mean±SEM for the number of determinations shown in parentheses. *p<0.05, **p<0.01, ***p<0.001 compared to their pre-hemorrhage baseline values.

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