Tuberc Respir Dis.  2006 Jan;60(1):49-56.

Antiinflammatory Effects of Heparin in Hemorrhage or LPS Induced Acute Lung Injury

• Kim JY
• Choi JC
• Lee YW
• Jung JW
• Shin JW
• Park IW
• Choi BW

Abstract

No abstract available.

MeSH Terms

Acute Lung Injury*
Hemorrhage*
Heparin*
Heparin

Figure

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  Figure 1 Design of the experiment. Mice were divided into 6 groups and each group was composed of 5 mice. Nonanticoagulant heparin (NCH, 6.25 mg/ml, 200 µl, 50 mg/kg) or PBS (200 µl) was injected into the tail vein. Immediately after the injection of NCH or PBS, hemorrhage or LPS-injection was followed. One hour after the induction of hemorrhage or LPS-injection, mice were anesthetized and lungs were removed for the evaluation of cytokines, MPO assay and wet-to-dry ratio.

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  Figure 2 NCH treatment did not reduce TNF-α expressions in the lung after LPS injection. Mice were injected with either PBS (200 µl) or NCH (50 mg/kg). Hemorrhage or LPS-injection (1 mg/kg, ip) was followed immediately. One hour after the induction of hemorrhage of LPS-injection, mice were anesthetized and the expression of TNF-α was evaluated by ELISA method. *; <0.05. PBS; phosphate buffered saline, NCH; nonanticoagulant heparin, PBS/ Hmr; PBS injection followed by hemorrhage, NCH/ Hmr; NCH injection followed by hemorrhage, PBS/ LPS; PBS injection followed by LPS injection, NCH/ LPS; NCH injection followed by LPS injection

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  Figure 3 NCH treatment did not reduce MIP-2 expressions in the lung after LPS injection. Mice were injected with either PBS (200 µl) or NCH (50 mg/kg). Hemorrhage or LPS-injection (1 mg/kg, ip) was followed immediately. One hour after the induction of hemorrhage of LPS-injection, mice were anesthetized and the expression of MIP-2 was evaluated by ELISA method. *; <0.05

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  Figure 4 NCH treatment did not reduce IL-1β expressions in the lung after LPS injection. Mice were injected with either PBS (200 µl) or NCH (50 mg/kg). Hemorrhage or LPS-injection (1 mg/kg, ip) was followed immediately. One hour after the induction of hemorrhage of LPS-injection, mice were anesthetized and the expression of IL-1β was evaluated by ELISA method. *; <0.05

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  Figure 5 NCH treatment did not reduce neutrophil recruitment in the lung after LPS injection. Mice were injected with either PBS (200 µl) or NCH (50 mg/kg). Hemorrhage or LPS-injection (1 mg/kg, ip) was followed immediately. One hour after the induction of hemorrhage of LPS-injection, mice were anesthetized and lung neutrophil recruitment was evaluated by MPO assay. *; <0.05

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  Figure 6 Either hemorrhage nor LPS-injection did not increase lung leakage at one hour after insults. Mice were injected with either PBS (200 µl) or NCH (50 mg/kg). Hemorrhage or LPS-injection (1 mg/kg, ip) was followed immediately. One hour after the induction of hemorrhage of LPS-injection, mice were anesthetized and lung leakage was evaluated by wet-to-dry ratio.

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