Yeungnam Univ J Med.  2002 Jun;19(1):55-62. 10.12701/yujm.2002.19.1.55.

Effect of Neutrophil Elastase inhibitor, ICI 200,355, on Interleukin-1 Induced acute lung injury in rats

• Chung JH
• Mun YC
• Park HJ
• Shin KC
• Lee KH

• KMID: 1909947
• DOI: http://doi.org/10.12701/yujm.2002.19.1.55

Abstract

BACKGROUND: Interleukin-1 (IL-1) and neutrophil appear to contribute to the pathogenesis of acute respiratory distress syndrome (ARDS). Elastase, as well as reactive oxygen species released from activated neutrophil, are thought to play pivotal roles in the experimental models of acute lung leak. This study investigated whether ICI 200,355, a synthetic elastase inhibitor, can attenuate acute lung injury induced by IL-1 in rats.
MATERIALS AND METHODS
We intratracheally instilled either saline or IL-1 with and without treatment of ICI 200,355 in rats. Lung lavage neutrophils, lung lavage cytokine-induced neutrophil chemoattractant(CINC) concentration, lung lavage protein concentration, lung myeloperoxidase(MPO) activity and lung leak index were measured at 5 hours of intratracheal treatment.
RESULTS
In rats given IL-1 intratracheally, lung lavage neutrophils, lung lavage CINC concentration, lung lavage protein concentration, lung MPO activity and lung leak index were higher. Intratracheal ICI 200,355 administration decreased lung lavage neutrophils, lung MPO activity and lung leak index, respectively, but did not decreased lung lavage CINC concentration.
CONCLUSION
These results suggest that ICI 200,355 decreases lung inflammation and leak without decreasing lung lavage CINC concentration in rats given IL-1 intratracheally.

Keyword

Acute lung injury; Interleukin-1; Elastase; ICI 200,355

MeSH Terms

Acute Lung Injury*
Animals
Bronchoalveolar Lavage
Interleukin-1*
Leukocyte Elastase*
Lung
Models, Theoretical
Neutrophils*
Pancreatic Elastase
Pneumonia
Rats*
Reactive Oxygen Species
Respiratory Distress Syndrome, Adult
Interleukin-1
Leukocyte Elastase
Pancreatic Elastase
Reactive Oxygen Species