Tuberc Respir Dis.  1996 Feb;43(1):8-13.

The Relationship between Isoniazid Resistance and 463 CodonMutation of katG Gne in Mycobacterium Tuberculosis

Affiliations
  • 1Korean Institute of Tuberculosis, Korean National Tuberculosis Association, Seoul, Korea.

Abstract

BACKGROUND
The 463 codon mutation of katG gene has been reported as an useful marker for the detection of isoniazid(INH) resistant strains of M. tuberculosis. This study aimed to elucidate relationship between 463 mutation in katG gene and INH resistance in M. tuberculosis. METHOD: DNA was extracted from 28 INH susceptible strains(MIC > or = 0.2microg/ml on the Lowenstein Jensen media) and used for amplification of 189bp fragment containing 463 codon by PCR. Amplified fragments were digested by restriction enzyme Msp I, analyzed by single strand conformation polymorphism(SSCP) in the MDE gel and sequenced to prove mutation. RESULT: Only 7(25%) out of 28 were digestible by restriction enzyme Msp I. The SSCP pattern of 21 strains were distinctly different from that of M. tuberculosis H37Rv. Msp I undigestible PCR fragment was substituted at 463 codon from Arg(CGG) to Leu(CTG).
CONCLUSION
This finding clearly indicate no relationship between 463 codon mutation of the katG gene and INH resistance.

Keyword

Mycobacterium tuberculosis; isoniazid; katG gene

MeSH Terms

Codon
DNA
Isoniazid*
Mycobacterium tuberculosis*
Mycobacterium*
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Tuberculosis
Codon
DNA
Isoniazid
Full Text Links
  • TRD
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr