Toxicol Res.  2013 Dec;29(4):249-255.

Genotoxicity Assessment of Erythritol by Using Short-term Assay

• Chung YS
• Lee M

Abstract

Erythritol is a sugar alcohol that is widely used as a natural sugar substitute. Thus, the safety of its usage is very important. In the present study, short-term genotoxicity assays were conducted to evaluate the potential genotoxic effects of erythritol. According to the OECD test guidelines, the maximum test dose was 5,000 microg/plate in bacterial reverse mutation tests, 5,000 microg/ml in cell-based assays, and 5,000 mg/kg for in vivo testing. An Ames test did not reveal any positive results. No clastogenicity was observed in a chromosomal aberration test with CHL cells or an in vitro micronucleus test with L5178Y tk +/- cells. Erythritol induced a marginal increase of DNA damage at two high doses by 24 hr of exposure in a comet assay using L5178Y tk +/- cells. Additionally, in vivo micronucleus tests clearly demonstrated that oral administration of erythritol did not induce micronuclei formation of the bone marrow cells of male ICR mice. Taken together, our results indicate that erythritol is not mutagenic to bacterial cells and does not cause chromosomal damage in mammalian cells either in vitro or in vivo.

Keyword

Erythritol; Ames assay; Chromosomal aberration test; Micronucleus assay; Comet assay; Genotoxicity

MeSH Terms

Administration, Oral
Animals
Bone Marrow Cells
Chromosome Aberrations
Comet Assay
DNA Damage
Erythritol*
Humans
Male
Mice
Mice, Inbred ICR
Micronucleus Tests
Sweetening Agents
Erythritol
Sweetening Agents