J Nutr Health.  2014 Apr;47(2):99-105. 10.4163/jnh.2014.47.2.99.

Effects of short-term supplementation of erythritol-salt on urinary electrolyte excretion in rats

• Kyung M
• Lim JY
• Lee K
• Jung S
• Choe K
• Yang CK
• Kim Y

• KMID: 2210023
• DOI: http://doi.org/10.4163/jnh.2014.47.2.99

Abstract

PURPOSE
This study was conducted in order to investigate the diuretic effects of Erythritol (ET) salt on urinary electrolyte excretion in Sprague-Dawley Rats.
METHODS
Animals were divided into two groups: Salt group (n = 7) and Salt + ET fed group (n = 7). Animals were provided food and water ad libitum. Supplements were administered orally to animals for one week.
RESULTS
Body weights were not statistically different between groups either on Day 1 or Day 7. However, water consumption of the Salt + ET group was significantly higher than that of the Salt group on Day 1 and Day 7. Urine volume of the Salt + ET group was approximately 27% and 38% higher than that of the Salt group on Day 1 and Day 7. In addition, we found that the total amounts of urinary electrolytes, such as sodium and potassium, of the Salt + ET group were significantly higher than those of the Salt group on Day 7. We also found that serum electrolyte concentrations did not differ between two groups. These results demonstrated that salt intake with ET was effective in increasing urinary electrolyte excretion, which might be caused by higher water intake and diuretic effect inhibiting reabsorption of water, sodium, and potassium in renal tubules.
CONCLUSION
The results suggest that short-term supplementation of ET salt can be a potential diuretic agent by inhibiting sodium and potassium reabsorption and inducing loss of water.

Keyword

erythritol; salt; electrolyte excretion; diuretic effect; hypertension

MeSH Terms

Animals
Body Weight
Diuretics
Drinking
Electrolytes
Erythritol
Hypertension
Potassium
Rats*
Rats, Sprague-Dawley
Sodium
Water
Diuretics
Electrolytes
Erythritol
Potassium
Sodium
Water

Figure

• 

  Fig. 1 Effects of the Erythritol-Salt on serum concentration of sodium and potassium. A: The serum concentrtion of sodium in two groups was measured at Day 7. B: The serum concentration of potassium in two groups was measured at Day 7. Values are expressed as mean ± SD (n = 7). *: significantly different from Salt group value (p < 0.05).

Reference

1. Intersalt Cooperative Research Group. Intersalt: an international study of electrolyte excretion and blood pressure. Results for 24 hour urinary sodium and potassium excretion. BMJ. 1988; 297(6644):319–328.

2. Kim YM, Byun JY, Han CK, Sung KS, Namgung B. Effects of organic salts fortified with seaweed components on blood pressure, serum minerals and hematochemicals in spontaneously hypertensive and normotensive rats. Korean J Food Sci Technol. 2009; 41(2):196–202.

3. Kim YM, Byun JY, Han CK, Sung KS, Namgung B. Effects of the Hizikia mineral salts on blood pressure and mineral metabolisms in spontaneously hypertensive rats and normotensive rats. Korean J Food Sci Technol. 2009; 41(2):203–209.

4. Statistics Korea. Annual report on the cause of death statistics. Daejeon: Statistics Korea;2011.

5. Yon M, Lee Y, Kim D, Lee J, Koh E, Nam E, Shin H, Kang BW, Kim JW, Heo S, Cho HY, Kim CI. Major sources of sodium intake of the Korean population at prepared dish level: based on the KNHANES 2008 & 2009. Korean J Community Nutr. 2011; 16(4):473–487.

6. Dahl LK. Role of dietary sodium in essential hypertension. J Am Diet Assoc. 1958; 34(6):585–590.

7. Kim IS, Seo ES, Jeon SY. A study on contents of salt in stored foods which homemakers prepared and their urine in Chon-buk province. Korean J Food Nutr. 1994; 7(3):183–191.

8. Kim HL, Park SM, Cho GS, Kim KY, Kim IC. Physicochemical characteristics, antimicrobial activity, ACE inhibitory activity of Chitosan-salt, and its antihypertensive effect. Food Sci Biotechnol. 2010; 19(3):777–784.

9. Ledingham JM. The distribution of water, sodium, and potassium in heart and skeletal muscle in experimental renal hypertension in rats. Clin Sci (Lond). 1953; 12(4):337–349.

10. Tobian L Jr, Binion J. Artery wall electrolytes in renal and DCA hypertension. J Clin Invest. 1954; 33(10):1407–1414.

11. Pohlová I, Zicha J. The influence of high salt intake and/or chronic blood volume expansion on renin-angiotensin system in Brattleboro rats. Acta Physiol Scand. 1992; 145(2):115–120.

12. Gupta BN, Linden RJ, Mary DA, Weatherill D. The influence of high and low sodium intake on blood volume in the dog. Q J Exp Physiol. 1981; 66(2):117–128.

13. Dustan HP, Bravo EL, Tarazi RC. Volume-dependent essential and steroid hypertension. Am J Cardiol. 1973; 31(5):606–615.

14. Ventura RR, Gomes DA, Reis WL, Elias LL, Castro M, Valença MM, Carnio EC, Rettori V, McCann SM, Antunes-Rodrigues J. Nitrergic modulation of vasopressin, oxytocin and atrial natriuretic peptide secretion in response to sodium intake and hypertonic blood volume expansion. Braz J Med Biol Res. 2002; 35(9):1101–1109.

15. Ministry of Health and Welfare, Korea Centers for Disease Control and Prevention. Korea Health Statistics 2009: Korea National Health and Nutrition Examination Survey (KNHANES IV-3). Cheongwon: Korea Centers for Disease Control and Prevent;2010.

16. He FJ, MacGregor GA. Reducing population salt intake worldwide: from evidence to implementation. Prog Cardiovasc Dis. 2010; 52(5):363–382.

17. Hagiwara SY, Takahashi M, Shen Y, Kaihou S, Tomiyama T, Yazawa M, Tamai Y, Sin Y, Kazusaka A, Terazawa M. A phytochemical in the edible Tamogi-take mushroom (Pleurotus cornucopiae), D-mannitol, inhibits ACE activity and lowers the blood pressure of spontaneously hypertensive rats. Biosci Biotechnol Biochem. 2005; 69(8):1603–1605.

18. Gongwer LE. Unpublished report submitted to WHO by Atlas Chemical Industries, Ltd. San Diego (CA): Atlas Chemical Industries, Ltd.;1963.

19. Bernt WO, Borzelleca JF, Flamm G, Munro IC. Erythritol: a review of biological and toxicological studies. Regul Toxicol Pharmacol. 1996; 24(2 Pt 2):S191–S197.

20. Dubernet MO, Bertrand A, Ribéreau-Gayon P. Constant presence in wines of erythritol, arabitol and mannitol. C R Acad Sci Hebd Seances Acad Sci D. 1974; 279(18):1561–1564.

21. Pitkaenen E, Pitkaenen A. Polyhydric alcohols in human urine. II. Ann Med Exp Biol Fenn. 1964; 42:113–116.

22. Hiele M, Ghoos Y, Rutgeerts P, Vantrappen G. Metabolism of erythritol in humans: comparison with glucose and lactitol. Br J Nutr. 1993; 69(1):169–176.

23. Noda K, Nakayama K, Oku T. Serum glucose and insulin levels and erythritol balance after oral administration of erythritol in healthy subjects. Eur J Clin Nutr. 1994; 48(4):286–292.

24. Bornet FR, Blayo A, Dauchy F, Slama G. Plasma and urine kinetics of erythritol after oral ingestion by healthy humans. Regul Toxicol Pharmacol. 1996; 24(2 Pt 2):S280–S285.

25. Munro IC, Berndt WO, Borzelleca JF, Flamm G, Lynch BS, Kennepohl E, Bär EA, Modderman J. Erythritol: an interpretive summary of biochemical, metabolic, toxicological and clinical data. Food Chem Toxicol. 1998; 36(12):1139–1174.

26. Bornet FR, Blayo A, Dauchy F, Slama G. Gastrointestinal response and plasma and urine determinations in human subjects given erythritol. Regul Toxicol Pharmacol. 1996; 24(2 Pt 2):S296–S302.

27. Til HP, Kuper CF, Falke HE, Bär A. Subchronic oral toxicity studies with erythritol in mice and rats. Regul Toxicol Pharmacol. 1996; 24(2 Pt 2):S221–S231.

28. Kanai M, Yamamoto H, Takahashi T, Onishi T, Shigeki Y. A 4-week feeding toxicity study on erythritol in rats with reduced renal function. Unpublished report from Division of Toxicology, Ohmiya Research Laboratory, Nikken Chemicals Co., Ltd., Japan (internal report). Saitama: Ohmiya Research Laboratory;1992.

29. Shibata M, Yamamoto S, Takahashi K, Kitamura S, Ichikawa N. Study on increased BUN caused by repeated administration of erythritol in rats. Division of Toxicology, Ohmiya Research Laboratory, Nikken Chemicals Co., Ltd., Japan (internal report). Saitama: Ohmiya Research Laboratory;1991.

30. Oku T, Noda K. Influence of chronic ingestion of newly developed sweetener, erythritol on growth and gastrointestinal function of the rats. Nutr Res. 1990; 10(9):987–996.

31. Til HP, Modderman J. Four-week oral toxicity study with erythritol in rats. Regul Toxicol Pharmacol. 1996; 24(2 Pt 2):S214–S220.

32. Montejano-Rodríguez JR, Almaguer-Vargas G, Gayosso-De-Lucio JA, Ocharan Hernández ME, Moreno Martínez RE, Hernández Caballero ME, Torres-Valencia JM, Sierra Ramírez JA. Evaluation of the diuretic activity of the ethanolic extract of Geranium seemannii Peyr. in Wistar rats. J Pharm Res. 2013; 6(7):709–713.

33. Fordtran JS, Rector FC Jr, Ewton MF, Soter N, Kinney J. Permeability characteristics of the human small intestine. J Clin Invest. 1965; 44(12):1935–1944.

34. Dean I, Jackson F, Greenough RJ. Chronic (1-year) oral toxicity study of erythritol in dogs. Regul Toxicol Pharmacol. 1996; 24(2 Pt 2):S254–S260.

35. The National BioResource Project for the Rat (JP). Japan: 2002-2014 NBRP rat Kyoto-Urine parameters [Internet]. [place unknown]: The National BioResource Project for the Rat;2014. cited 2012 June 15. Available from: http://www.anim.med.kyoto-u.ac.jp/nbr/strainsx/urine_list.aspx.

36. Lin YH, Wang SM, Wu VC, Lee JK, Kuo CC, Yen RF, Liu KL, Huang KH, Chueh SC, Wang WJ, Lin LY, Chien KL, Ho YL, Chen MF, Wu KD. TAIPAI study group. The association of serum potassium level with left ventricular mass in patients with primary aldosteronism. Eur J Clin Invest. 2011; 41(7):743–750.