Restor Dent Endod.  2014 May;39(2):95-103.

Pull-out bond strength of a self-adhesive resin cement to NaOCl-treated root dentin: effect of antioxidizing agents

• Khoroushi
• Kachuei M

Abstract

OBJECTIVES
This study evaluated the effect of three antioxidizing agents on pull-out bond strengths of dentin treated with sodium hypochlorite.
MATERIALS AND METHODS
Root canals of 75 single-rooted human teeth were prepared. Fifteen teeth were irrigated with normal saline for a negative control group, and the remaining 60 teeth (groups 2 - 5) with 2.5% NaOCl. The teeth in group 2 served as a positive control. Prior to post cementation, the root canals in groups 3 - 5 were irrigated with three antioxidizing agents including 10% rosmarinic acid (RA, Baridge essence), 10% hesperidin (HPN, Sigma), and 10% sodium ascorbate hydrogel (SA, AppliChem). Seventy-five spreaders (#55, taper .02, Produits Dentaires S.A) were coated with silica and silanized with the Rocatec system and ceramic bond. All the prepared spreaders were cemented with a self-adhesive resin cement (Bifix SE, Voco Gmbh) in the prepared canals. After storage in distilled water (24 h/37degrees C), the spreaders were pulled out in a universal testing machine at a crosshead speed of 1.0 mm/min. Pull-out strength values were analyzed by one-way ANOVA and Tukey's HSD test (alpha = 0.05).
RESULTS
There were significant differences between study groups (p = 0.016). The highest pull-out strength was related to the SA group. The lowest strength was obtained in the positive control group.
CONCLUSIONS
Irrigation with NaOCl during canal preparation decreased bond strength of resin cement to root dentin. Amongst the antioxidants tested, SA had superior results in reversing the diminishing effect of NaOCl irrigation on the bond strength to root dentin.

Keyword

Antioxidant; Bond strength; Endodontically treated teeth; Resin cement

MeSH Terms

Antioxidants
Ascorbic Acid
Cementation
Ceramics
Dental Pulp Cavity
Dentin*
Hesperidin
Humans
Hydrogel
Resin Cements*
Silicon Dioxide
Sodium Hypochlorite
Tooth
Water
Antioxidants
Ascorbic Acid
Ceramics
Hesperidin
Hydrogel
Resin Cements
Silicon Dioxide
Sodium Hypochlorite
Water

Figure

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  Figure 1 The SEM photomicrographs of group 1. C, cement resin; D, dentin; P, post (Magnification: both ×500).

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  Figure 2 The SEM photomicrographs of group 2. C, cement resin; D, dentin; (Magnifications: ×500 and ×2,500, respectively).

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  Figure 3 The SEM photomicrographs of group 3. C, cement resin; D, dentin; (Magnifications: ×500 and ×2,500, respectively).

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  Figure 4 The SEM photomicrographs of group 4. C, cement resin; D, dentin (Magnifications: ×500 and ×2,500, respectively).

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  Figure 5 The SEM photomicrographs of group 5. C, cement resin; D, dentin; P, post (Magnifications: ×500 and ×2,500, respectively). As it appears in the photomicrographs, the resin interface area seems to be wider in groups 2 and 3. There is a much better adaptation between the resin cement and root dentin in groups 1, 4, and 5. In group 5, the resin tags apparently are larger and more numerous.

Reference

1. Morgano SM. Restoration of pulpless teeth: application of traditional principles in present and future contexts. J Prosthet Dent. 1996; 75:375–380.

2. Ebert J, Leyer A, Günther O, Lohbauer U, Petschelt A, Frankenberger R, Roggendorf MJ. Bond strength of adhesive cements to root canal dentin tested with a novel pull-out approach. J Endod. 2011; 37:1558–1561.

3. Glazer B. Restoration of endodontically treated teeth with carbon fibre posts-a prospective study. J Can Dent Assoc. 2000; 66:613–618.

4. Malferrari S, Monaco C, Scotti R. Clinical evaluation of teeth restored with quartz fiber-reinforced epoxy resin posts. Int J Prosthodont. 2003; 16:39–44.

5. Amaral M, Santini MF, Wandscher V, Amaral R, Valandro LF. An in vitro comparison of different cementation strategies on the pull-out strength of a glass fiber post. Oper Dent. 2009; 34:443–451.

6. Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, De Stefano Dorigo E. Dental adhesion review: aging and stability of the bonded interface. Dent Mater. 2008; 24:90–101.

7. Hashimoto M, Ohno H, Sano H, Kaga M, Oguchi H. In vitro degradation of resin-dentin bonds analyzed by microtensile bond test, scanning and transmission electron microscopy. Biomaterials. 2003; 24:3795–3803.

8. Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, Ito S. Collagen degradation by host-derived enzymes during aging. J Dent Res. 2004; 83:216–221.

9. Carrilho MR, Carvalho RM, de Goes MF, di Hipólito V, Geraldeli S, Tay FR, Pashley DH, Tjäderhane L. Chlorhexidine preserves dentin bond in vitro. J Dent Res. 2007; 86:90–94.

10. Chaussain-Miller C, Fioretti F, Goldberg M, Menashi S. The role of matrix metalloproteinases (MMPs) in human caries. J Dent Res. 2006; 85:22–32.

11. Hebling J, Pashley DH, Tjäderhane L, Tay FR. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res. 2005; 84:741–746.

12. Bedran-Russo AK, Yoo KJ, Ema KC, Pashley DH. Mechanical properties of tannic-acid-treated dentin matrix. J Dent Res. 2009; 88:807–811.

13. Macedo GV, Yamauchi M, Bedran-Russo AK. Effects of chemical cross-linkers on caries-affected dentin bonding. J Dent Res. 2009; 88:1096–1100.

14. Al-Ammar A, Drummond JL, Bedran-Russo AK. The use of collagen cross-linking agents to enhance dentin bond strength. J Biomed Mater Res B Appl Biomater. 2009; 91:419–424.

15. Wang R, Zhou W, Jiang X. Reaction kinetics of degradation and epimerization of epigallocatechin gallate (EGCG) in aqueous system over a wide temperature range. J Agric Food Chem. 2008; 56:2694–2701.

16. Magalhães AC, Wiegand A, Rios D, Hannas A, Attin T, Buzalaf MA. Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ. J Dent. 2009; 37:994–998.

17. Cho YH, Kim JH, Sim GS, Lee BC, Pyo HB, Park HD. Inhibitory effects of antioxidant constituents from Melothria heterophylla on matrix metalloproteinase-1 expression in UVA-irradiated human dermal fibroblasts. J Cosmet Sci. 2006; 57:279–289.

18. Islam SM, Hiraishi N, Nassar M, Sono R, Otsuki M, Takatsura T, Yiu C, Tagami J. In vitro effect of hesperidin on root dentin collagen and de/re-mineralization. Dent Mater J. 2012; 31:362–367.

19. Guardia T, Rotelli AE, Juarez AO, Pelzer LE. Antiinflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Farmaco. 2001; 56:683–687.

20. Trzeciakiewicz A, Habauzit V, Mercier S, Barron D, Urpi-Sarda M, Manach C, Offord E, Horcajada MN. Molecular mechanism of hesperetin-7-O-glucuronide, the main circulating metabolite of hesperidin, involved in osteoblast differentiation. J Agric Food Chem. 2010; 58:668–675.

21. Nostro A, Cannatelli MA, Crisafi G, Musolino AD, Procopio F, Alonzo V. Modifications of hydrophobicity, in vitro adherence and cellular aggregation of Streptococcus mutans by Helichrysum italicum extract. Lett Appl Microbiol. 2004; 38:423–427.

22. Hiraishi N, Sono R, Islam MS, Otsuki M, Tagami J, Takatsuka T. Effect of hesperidin in vitro on root dentine collagen and demineralization. J Dent. 2011; 39:391–396.

23. Hirata A, Murakami Y, Shoji M, Kadoma Y, Fujisawa S. Kinetics of radical-scavenging activity of hesperetin and hesperidin and their inhibitory activity on COX-2 expression. Anticancer Res. 2005; 25:3367–3374.

24. Benavente-García O, Castillo J. Update on uses and properties of citrus flavonoids: new findings in anticancer, cardiovascular, and anti-inflammatory activity. J Agric Food Chem. 2008; 56:6185–6205.

25. Miller EG, Peacock JJ, Bourland TC, Taylor SE, Wright JM, Patil BS, Miller EG. Inhibition of oral carcinogenesis by citrus flavonoids. Nutr Cancer. 2008; 60:69–74.

26. Wood N. Bound sugars in hepatic glycoproteins from male rats during dietary citrus bioflavonoid and/or ascorbic acid supplementation. J Med Food. 2005; 8:512–517.

27. Horcajada MN, Habauzit V, Trzeciakiewicz A, Morand C, Gil-Izquierdo A, Mardon J, Lebecque P, Davicco MJ, Chee WS, Coxam V, Offord E. Hesperidin inhibits ovariectomized-induced osteopenia and shows differential effects on bone mass and strength in young and adult intact rats. J Appl Physiol. 2008; 104:648–654.

28. Choi EM, Kim YH. Hesperetin attenuates the highly reducing sugar-triggered inhibition of osteoblast differentiation. Cell Biol Toxicol. 2008; 24:225–231.

29. Islam S, Hiraishi N, Nassar M, Yiu C, Otsuki M, Tagami J. Effect of natural cross-linkers incorporation in a self-etching primer on dentine bond strength. J Dent. 2012; 40:1052–1059.

30. Lyubimova T, Caglio S, Gelfi C, Righetti PG, Rabilloud T. Photopolymerization of polyacrylamide gels with methylene blue. Electrophoresis. 1993; 14:40–50.

31. Tirkeş S, Toppare L, Alkan S, Bakir U, Onen A, Yağci Y. Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers. Int J Biol Macromol. 2002; 30:81–87.

32. Hernández-Hernández E, Ponce-Alquicira E, Jaramillo-Flores ME, Guerrero Legarreta I. Antioxidant effect rosemary (Rosmarinus officinalis L.) and oregano (Origanum vulgare L.) extracts on TBARS and colour of model raw pork batters. Meat Sci. 2009; 81:410–417.

33. Apak R, Güçlü K, Ozyürek M, Bektas Oğlu B, Bener M. Cupric ion reducing antioxidant capacity assay for food antioxidants: vitamins, polyphenolics, and flavonoids in food extracts. Methods Mol Biol. 2008; 477:163–193.

34. Bowen RL. Adhesive bonding of various materials to hard tooth tissues. IV. Bonding to dentin, enamel, and fluorapatite improved by the use of a surface-active comonomer. J Dent Res. 1965; 44:906–911.

35. Taniguchi G, Nakajima M, Hosaka K, Iwamoto N, Ikeda M, Foxton RM, Tagami J. Improving the effect of NaOCl pretreatment on bonding to caries-affected dentin using self-etch adhesives. J Dent. 2009; 37:769–775.

36. Prasansuttiporn T, Nakajima M, Kunawarote S, Foxton RM, Tagami J. Effect of reducing agents on bond strength to NaOCl-treated dentin. Dent Mater. 2011; 27:229–234.

37. da Cunha LF, Furuse AY, Mondelli RF, Mondelli J. Compromised bond strength after root dentin deproteinization reversed with ascorbic acid. J Endod. 2010; 36:130–134.

38. Weston CH, Ito S, Wadgaonkar B, Pashley DH. Effects of time and concentration of sodium ascorbate on reversal of NaOCl-induced reduction in bond strengths. J Endod. 2007; 33:879–881.

39. Moreira DM, de Andrade Feitosa JP, Line SR, Zaia AA. Effects of reducing agents on birefringence dentin collagen after use of different endodontic auxiliary chemical substances. J Endod. 2011; 37:1406–1411.

40. May LG, Salvia AC, Souza RO, Michida SM, Valera MC, Takahashi FE, Bottino MA. Effect of sodium ascorbate and the time lapse before cementation after internal bleaching on bond strength between dentin and ceramic. J Prosthodont. 2010; 19:374–380.

41. Bouillaguet S, Troesch S, Wataha JC, Krejci I, Meyer JM, Pashley DH. Microtensile bond strength between adhesive cements and root canal dentin. Dent Mater. 2003; 19:199–205.

42. Feilzer AJ, De Gee AJ, Davidson CL. Increased wall-to-wall curing contraction in thin bonded resin layers. J Dent Res. 1989; 68:48–50.

43. Kremeier K, Fasen L, Klaiber B, Hofmann N. Influence of endodontic post type (glass fiber, quartz fiber or gold) and luting material on push-out bond strength to dentin in vitro. Dent Mater. 2008; 24:660–666.

44. Park JY, Kwon TY, Kim YK. Effective application duration of sodium ascorbate antioxidant in reducing microleakage of bonded composite restoration in intracoronally-bleached teeth. Restor Dent Endod. 2013; 38:43–47.

45. Hikita K, Van Meerbeek B, De Munck J, Ikeda T, Van Landuyt K, Maida T, Lambrechts P, Peumans M. Bonding effectiveness of adhesive luting agents to enamel and dentin. Dent Mater. 2007; 23:71–80.

46. Kim DS, Park SH, Choi GW, Choi KK. The effect of bonding resin on bond strength of dual-cure resin cements. J Korean Acad Conserv Dent. 2007; 32:426–436.

47. Kim SR, Yum J, Park JK, Hur B, Kim HC. Comparison of push-out bond strength of post according to cement application methods. J Korean Acad Conserv Dent. 2010; 35:479–485.

48. Khoroushi M, Saneie T. Post-bleaching application of an antioxidant on dentin bond strength of three dental adhesives. Dent Res J (Isfahan). 2012; 9:46–53.

49. Khoroushi M, Aghelinejad S. Effect of postbleaching application of an antioxidant on enamel bond strength of three different adhesives. Med Oral Patol Oral Cir Bucal. 2011; 16:e990–e996.

50. Miguez PA, Pereira PN, Atsawasuwan P, Yamauchi M. Collagen cross-linking and ultimate tensile strength in dentin. J Dent Res. 2004; 83:807–810.