Psychiatry Investig.
2014 Jul;11(3):313-318.
NCAM140 and pCREB Expression after Tianeptine Treatment of SH-SY5Y Cells
- Affiliations
-
- 1Department of General Psychiatry, Seoul National Hospital, Seoul, Republic of Korea.
- 2Cognitive Neuroscience and Schizophrenia Program, Nathan Kline Institute, Orangeburg, New York, USA.
- 3Department of Neuropsychiatry, College of Medicine, Hanyang University, Hanyang University Guri Hospital, Guri, Republic of Korea. jchoi@hanyang.ac.kr
- 4Division of Molecular and Life Sciences, Hanyang University, Ansan, Republic of Korea.
- 5Department of Neuropsychiatry and Mental Health Institute, College of Medicine, Hanyang University, Seoul, Republic of Korea.
Abstract
OBJECTIVE
Antidepressants Modulate Neuronal Plasticity. Tianeptine, An Atypical Antidepressant, Might Be Involved In The Restoration Of Neuronal Plasticity; It Primarily Enhances The Synaptic Reuptake Of Serotonin. Ncam140 Is Involved In Neuronal Development Processes, Synaptogenesis And Synaptic Plasticity. We Investigated The Effect Of Tianeptine On The Expression Of Ncam140 And Its Downstream Signaling Molecule In The Human Neuroblastoma Cell Line Sh-sy5y.
METHODS
NCAM protein expression was measured in human neuroblastoma SH-SY5Y cells that were cultivated in serum-free media and treated with 0, 10, or 20 microM tianeptine for 6, 24, or 72 hours. NCAM140 expression in the tianeptine treatment group was confirmed by Western blot, and quantified through measurement of band intensity by absorbance. CREB and pCREB expression was identified after treatment with 20 microM tianeptine for 6, 24, and 72 hours by Western blot.
RESULTS
Compared to cells treated for 6 hours, cells treated with 0 or 10 microM tianeptine for 72 hours showed a significant increase in NCAM140 expression and cells treated with 20 microM tianeptine showed a significant increase after 24 and 72 hours. The pCREB level in cells treated with 20 microM tianeptine increased in time-dependent manner.
CONCLUSION
Our findings indicated that the tianeptine antidepressant effect may occur by induction of NCAM140 expression and CREB phosphorylation.
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