Pediatr Allergy Respir Dis.
2009 Dec;19(4):345-353.
Quantitative Analysis of Egg Protein by ELISA in Distributed Influenza Vaccine in Korea
- Affiliations
-
- 1Department of Pediatrics, College of Medicine, Konyang University, Daejeon, Korea.
- 2Department of Pediatrics, College of Medicine Dankook University Hospital, Cheonan, Korea. ehchung@dankook.ac.kr
- 3Department of Laboratory Medicine, College of Medicine Dankook University Hospital, Cheonan, Korea.
Abstract
- PURPOSE
Influenza infection is a major public health concern, especially in young children and in high risk groups such as asthma patients. Traditionally, influenza vaccines are produced from infertilized eggs. Therefore, patients with severe egg allergy can show adverse reactions after vaccination and be dalayed in active immunization. The aim of this study was to determine the ovalbumin concentration of a commercially available influenza vaccines in Korea.
METHODS
The ovalbumin content in each vaccine was determined by enzyme linked immunosorbent assay. The ovalbumin content was compared among the different manufactures, the different lot numbers and the same lot numbers of vaccines. The microtiter plates coated with goat anti-chicken egg albumin antibody were inoculated with serial diluted vaccines and ovalbumin. After incubation with horse radish peroxidase-conjugate rabbit anti-ovalbumin, absorbance was read at 450 nm and fitted to the standard curve.
RESULTS
The highest ovalbumin content was 101.24 ng/mL, and the lowest ovalbumin content was 21.23 ng/mL. The amount of ovalbumin differed not only among the different vaccines, but also different lot numbers of individual vaccines and even the same lot numbers of vaccines.
CONCLUSION
In none of the vaccines tested in our study, ovalbumin content reached the maximum values for ovalbumin (1.2 microgram/mL). Therefore, in severe egg allergic patients, our results provide the useful information on influenza vaccination.
