Pediatr Allergy Respir Dis.
2007 Dec;17(4):334-353.
Viral Etiologic Agents in Acute Viral Lower Respiratory Tract Detected by Multiplex RT-PCR
- Affiliations
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- 1Department of Pediatrics, Soonchunhyang University Hospital, Soonchunhyang University School, Chungnam, Korea. allergist@korea.com
- 2Department of Preventive Medicine, Soonchunhyang University Hospital, Soonchunhyang University School, Chungnam, Korea.
- 3Department of Clinical Parasitology, Soonchunhyang University Hospital, Soonchunhyang University School, Chungnam, Korea.
- 4Department of Laboratory Medicine, Soonchunhyang University Hospital, Soonchunhyang University School, Chungnam, Korea.
- 5Department of Pathology, Soonchunhyang University Hospital, Soonchunhyang University School, Chungnam, Korea.
Abstract
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PURPOSE:This study was performed to examine seasonal occurrences of respiratory viruses and detect viral etiologic agents, by multiplex RT-PCR in children with acute lower respiratory tract infection (ALRI).
METHODS
Nasopharyngeal aspirates (NPA) were collected from 654 hospitalized children with ALRI on admission at Soonchunhyang University Cheonan Hospital and analysed by multiplex RT-PCR between January 2005 and December 2005.
RESULTS
Viral agents were detected from 234 subjects (35.8%). The identified viral pathogens were respiratory syncytial virus (RSV) (34.2%), adenovirus (33.8%), parainfluenza virus (21.8%), influenza virus type A (8.1%) and influenza virus type B (2.1%). Viral ALRI occurred most frenquently during the 1st year of life. Clinical diagnoses of viral ALRI included pneumonia (72.2%), bronchiolitis (14.5%), tracheobronchitis (9.8%) and croup (3.5%). The most common cause of bronchiolitis was RSV. Common clinical symptoms and signs of viral ALRI were cough (93.4%), fever (78.2%), rhinorrhea (72.5%), rale (60.2%), and wheezing (26.2%).
CONCLUSION
Viral agents were detected by multiplex RT-PCR in 35.8% of NPA obtained from 654 hospitalized patients with acute respiratory tract infections. Multiplex RT-PCR may be useful for detecting virus in children.
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