Pediatr Allergy Respir Dis.
1998 Sep;8(2):215-220.
Production of Recombinant Buckwheat Allergen
- Affiliations
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- 1Department of Pediatrics, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
Abstract
- PURPOSE
Buckwheat is one of the most common food allegen in Korea and frequently elicit severe allergic reactions. However, up to now, only few reports on buckwheat allergens have been reproted. The purpose of this study was to isolate mRNA for the production of recombinant buckwheat allergens.
METHODS
After the isolation of mRNA from ripening buckwheat seeds, in vitro translation was performed. The proteim patters of in vitro traslate products were identified using SDS-PAGE.
RESULTS
A260/A260 ratio total RNA was 1.9 and mRNA was 2.0. In vitro translate products showed towered molecular buckwheat proteins such as 1, 3, and 14kD, while other high molecular weight protein of buckwheat seed were not shown in SDS-PAGE.
CONCLUSION
mRNA of buckwheat was purified only from ripening seeds. The composition of mRNA was different according to the ripening periods. It is believed that this finding can give a clue to the basic research of buckwheat allergen.
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