Nutr Res Pract.  2015 Feb;9(1):17-21. 10.4162/nrp.2015.9.1.17.

Inhibitory effect of Erythronium japonicum on the human breast cancer cell metastasis

• You MK
• Kim MS
• Rhyu J
• Bang MA
• Kim HA

• KMID: 2313804
• DOI: http://doi.org/10.4162/nrp.2015.9.1.17

Abstract

BACKGROUND/OBJECTIVES
In this study, the inhibitory effect of Erythronium japonicum extracts on the metastasis of MDA-MB-231 human breast cancer cell line was determined.
MATERIALS/METHODS
Cells were cultured with DMSO or with 50, 75, 100 or 250 microg/ml of Erythronium japonicum methanol or ethanol extract.
RESULTS
Both methanol and ethanol extracts significantly inhibited the growth and induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Erythronium japonicum extracts inhibited the adhesion of MDA-MB-231 cells. The invasion of breast cancer cells was suppressed by Erythronium japonicum extracts in a dose-dependent manner. The motility and MMP-2 and MMP-9 activities were also inhibited by both methanol and ethanol extracts.
CONCLUSIONS
Our results collectively indicate that Erythronium japonicum extracts inhibit the growth, adhesion, migration and invasion as well as induce the apoptosis of human breast cancer cells. Clinical application of Erythronium japonicum as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.

Keyword

Erythronium japonicum; cancer; proliferation; apoptosis; invasion

MeSH Terms

Apoptosis
Breast Neoplasms*
Cell Line
Dimethyl Sulfoxide
Ethanol
Humans
Methanol
Neoplasm Metastasis*
Dimethyl Sulfoxide
Ethanol
Methanol

Figure

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  Fig. 1 Effects of Erythronium japonicum on the proliferation of MDA-MB-231 cells. C: Control; M: Methanol extract of Erythronium japonicum; E: Ethanol extract of Erythronium japonicum. Data are mean ± SD. Means with the same letter are not significantly different by Duncan's multiple range test (P < 0.05).

• 

  Fig. 2 Effects of Erythronium japonicum on the apoptosis of MDA-MB-231 cells. C: Control; M: Methanol extract of Erythronium japonicum; E: Ethanol extract of Erythronium japonicum. Data are mean ± SD. Means with the same letter are not significantly different by Duncan's multiple range test (P < 0.05).

• 

  Fig. 3 Effects of Erythronium japonicum on the adhesion of MDA-MB-231 cells. C: Control; M: Methanol extract of Erythronium japonicum; E: Ethanol extract of Erythronium japonicum. Data are mean ± SD. Means with the same letter are not significantly different by Duncan's multiple range test (P < 0.05).

• 

  Fig. 4 Effects of Erythronium japonicum on the invasion of MDA-MB-231 cells. C: Control; M: Methanol extract of Erythronium japonicum; E: Ethanol extract of Erythronium japonicum. Data are mean ± SD. Means with the same letter are not significantly different by Duncan's multiple range test (P < 0.05).

• 

  Fig. 5 Effects of Erythronium japonicum on the migration of MDA-MB-231 cells. C: Control; M: Methanol extract of Erythronium japonicum; E: Ethanol extract of Erythronium japonicum. 0 h & 24 h: hours after making wound

• 

  Fig. 6 Effects of Erythronium japonicum on the activities of MMP-2 and MMP-9 of MDA-MB-231 cells. C: Control; M: Methanol extract of Erythronium japonicum; E: Ethanol extract of Erythronium japonicum

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