Nutr Res Pract.  2012 Oct;6(5):381-388.

Protein quality, hematological properties and nutritional status of albino rats fed complementary foods with fermented popcorn, African locust bean, and bambara groundnut flour blends

Affiliations
  • 1Department of Food Science and Technology (Human Nutrition Division), Federal University of Technology, Akure, Ondo State, Nigeria. soijarotimi@gmail.com
  • 2Department of Human Nutrition, Faculty of Public Health, College of Medicine, University of Ibadan, Nigeria.

Abstract

The objective of this study was to determine protein quality and hematological properties of infant diets formulated from local food materials. The food materials were obtained locally, fermented, and milled into flour. The flours were mixed as 70% popcorn and 30% African locust bean (FPA), 70% popcorn and 30% bambara groundnut (FPB), and 70% popcorn, 20% bambara groundnut, and 10% African locust bean (FPAB). Proximate analysis, protein quality, hematological properties, and anthropometric measurements of the animals fed with the formulations were investigated. The protein contents of the formulated diets were significantly higher than that of Cerelac (a commercial preparation) (15.75 +/- 0.01 g/100 g) and ogi (traditional complementary food) (6.52 +/- 0.31 g/100 g). The energy value of FPAB (464.94 +/- 1.22 kcal) was higher than those of FPA (441.41 +/- 3.05 kcal) and FPB (441.48 +/- 3.05 kcal). The biological value (BV) of FPAB (60.20%) was the highest followed by FPB (44.24%) and FPA (41.15%); however, BV of the diets was higher than that of ogi (10.03%) but lower than that of Cerelac (70.43%). Net protein utilization (NPU) of the formulations was 41.16-60.20%, whereas true protein digestibility was 41.05-60.05%. Metabolizable energy (232.98 kcal) and digestible energy (83.69 kcal) of FPAB were the highest, whereas that of FPA had the lowest values. The protein digestibility values corrected for amino acid score of the diets (0.22-0.44) were lower than that of Cerelac (0.52), but higher than that of ogi (0.21). The growth patterns and hematological properties (packed cell volume, red blood cells, hemoglobin, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, and mean corpuscular volume) of the formulated diets were higher than those of ogi, but lower than those of Cerelac. In conclusion, we established that the FPAB food sample was rated best in terms of protein quality over the other formulated diets. Therefore, a FPAB blend may be used as a substitute for ogi.

Keyword

Complementary foods; protein quality; hematological properties

MeSH Terms

Animals
Cell Size
Diet
Erythrocyte Indices
Erythrocytes
Flour
Grasshoppers
Hemoglobins
Humans
Infant
Nutritional Status
Rats
Hemoglobins

Figure

  • Fig. 1 Comparison of protein digestibility using the corrected amino acid score (PDCAAS) of the formulated food samples, ogi (a traditional complementary food), Cerelac (a commercial formula), and casein (standard) (P < 0.05). FPA, fermented popcorn-African locust bean blend; FPB, fermented popcorn-Bambara groundnut blend; FPAB, fermented popcorn-African locust-Bambara groundnut blend.

  • Fig. 2 Amount of the formulated food samples required to meet total essential amino acid requirements compared with that of ogi (a traditional complementary food), Cerelac (a commercial formula), and casein (standard) (P < 0.05). FPA, fermented popcorn-African locust bean blend; FPB, fermented popcorn-Bambara groundnut blend; FPAB, fermented popcorn-African locust-Bambara groundnut blend.

  • Fig. 3 Weight-for-age in albino rats fed with the fermented formulated complementary foods compared with rats fed ogi (a traditional complementary food) and Cerelac (a commercial formula) (P < 0.05). FPA, fermented popcorn-African locust bean blend; FPB, fermented popcorn-Bambara groundnut blend; FPAB, fermented popcorn-African locust-Bambara groundnut blend.

  • Fig. 4 Lenght-for-age in albino rats fed the fermented formulated complementary foods compared with rats fed ogi (a traditional complementary food) and Cerelac (a commercial formula) (P < 0.05). FPA, fermented popcorn-African locust bean blend; FPB, fermented popcorn-Bambara groundnut blend; FPAB, fermented popcorn-African locust-Bambara groundnut blend.


Reference

1. Lutter C. Meeting the challenge to improve complementary feeding. SCN News. 2003. (27):4–9.
2. Lutter CK, Rivera JA. Nutritional status of infants and young children and characteristics of their diets. J Nutr. 2003. 133:2941S–2949S.
Article
3. Daelmans B, Saadeh R. Global initiatives to improve complementary feeding. SCN News. 2003. (27):10–18.
4. Anigo KM, Ameh DA, Ibrahim S, Danbauch S. Infant feeding practices and nutritional status of children in North Western Nigeria. Asian J Clin Nutr. 2009. 1:12–22.
Article
5. Pollitt E. Poverty and child development: relevance of research in developing countries to the United States. Child Dev. 1994. 65:283–295.
Article
6. Duncan GJ, Brooks-Gunn J, Klebanov PK. Economic deprivation and early childhood development. Child Dev. 1994. 65:296–318.
Article
7. Kretchmer N, Beard JL, Carlson S. The role of nutrition in the development of normal cognition. Am J Clin Nutr. 1996. 63:997S–1001S.
Article
8. Brown KH, Black RE, López de Romaña G, Creed de Kanashiro H. Infant-feeding practices and their relationship with diarrheal and other diseases in Huascar (Lima), Peru. Pediatrics. 1989. 83:31–40.
Article
9. Bhandari N, Bahl R, Taneja S, Strand T, Mølbak K, Ulvik RJ, Sommerfelt H, Bhan MK. Substantial reduction in severe diarrheal morbidity by daily zinc supplementation in young North Indian children. Pediatrics. 2002. 109:e86.
Article
10. Kalanda BF, Verhoeff FH, Brabin BJ. Breast and complementary feeding practices in relation to morbidity and growth in Malawian infants. Eur J Clin Nutr. 2006. 60:401–407.
Article
11. Edmond KM, Zandoh C, Quigley MA, Amenga-Etego S, Owusu-Agyei S, Kirkwood BR. Delayed breastfeeding initiation increases risk of neonatal mortality. Pediatrics. 2006. 117:e380–e386.
Article
12. Brown KH. The importance of dietary quality versus quantity for weanlings in less developed countries: a framework for discussion. Food Nutr Bull. 1991. 13:86–94.
Article
13. Golden MH. The nature of nutritional deficiency in relation to growth failure and poverty. Acta Paediatr Scand Suppl. 1991. 374:95–110.
Article
14. Naismith DJ. Kwashiorkor in Western Nigeria: a study of traditional weaning foods, with particular reference to energy and linoleic acid. Br J Nutr. 1973. 30:567–576.
Article
15. Fashakin JB, Ogunsola F. The utilization of local foods in the formulation of weaning foods. J Trop Pediatr. 1982. 28:93–96.
Article
16. Oyenuga VA. Nigeria's Foods and Feeding-Stuffs: Their Chemistry and Nutritive Value. 1968. Nigeria: Ibadan University Press;234–457.
17. Association of Official Analytical Chemists (AOAC). Official Methods of Analysis of the Association of Analytical Chemists International. 2005. 18th ed. Washington, D. C.: AOAC.
18. Pearson D. The Chemical Analysis of Foods. 1976. 6th ed. London: Churchill;6–7.
19. Phillips DE, Erye MD, Thompson A, Boulter D. Protein quality in seed meals of Phaseolus vulgaris and heat-stable factors affecting the utilisation of protein. J Sci Food Agric. 1981. 32:423–432.
Article
20. National Academy of Sciences (NAS). National Research Council (NCR). Evaluation of Protein Quality. 1963. Washington, D.C.: NAS/NCR;23–37.
21. Dreyer JJ. Biological assessment of protein quality: digestibility of the proteins in certain foodstuffs. S Afr Med J. 1968. 42:1304–1313.
22. McCance RA, Widdowson EM. Special Report Series No. 297. The Composition of Foods. 1960. London: Medical Research Council.
23. Göranzon H, Forsum E, Thilén M. Calculation and determination of metabolizable energy in mixed diets to humans. Am J Clin Nutr. 1983. 38:954–963.
Article
24. Lamb GM. Manual of Veterinary Laboratory Technique in Kenya. 1981. Kenya: Ciba-Geigy;96–102.
25. Griffith LD, Castell-Perez ME, Griffith ME. Effects of blend and processing method on the nutritional quality of weaning foods made from select cereals and legumes. Cereal Chem. 1998. 75:105–112.
Article
26. Bennett VA, Morales E, González J, Peerson JM, López de Romaña G, Brown KH. Effects of dietary viscosity and energy density on total daily energy consumption by young Peruvian children. Am J Clin Nutr. 1999. 70:285–291.
Article
27. Mosha TCE, Laswai HS, Tetens I. Nutritional composition and micronutrient status of home made and commercial weaning foods consumed in Tanzania. Plant Foods Hum Nutr. 2000. 55:185–205.
28. Amankwah EA, Barimah J, Acheampong R, Addai LO, Nnaji CO. Effect of fermentation and malting on the viscosity of maize-soyabean weaning blends. Pak J Nutr. 2009. 8:1671–1675.
Article
29. Walker AF. The contribution of weaning foods to protein-energy malnutrition. Nutr Res Rev. 1990. 3:25–47.
Article
30. McGuire J. Quality versus quantity of infant diets: translating research into action. Food Nutr Bull. 1991. 13:132–134.
Article
31. Food & Agriculture Organization of the United Nations (FAO). World Health Organization (WHO). FAO Food and Nutrition Paper 51. Protein Quality Evaluation: Report of the Joint FAO/WHO Expert Consultation. 1991. Rome: FAO.
32. Gilani GS, Sepehr E. Protein digestibility and quality in products containing antinutritional factors are adversely affected by old age in rats. J Nutr. 2003. 133:220–225.
Article
33. Abbey BW, Nkanga UB. Production of high quality weaning products from Maize-Cowpea-Crayfish mixtures. Nutr Rep Int. 1988. 37:951–957.
34. Michaelsen KF, Friis H. Complementary feeding: a global perspective. Nutrition. 1998. 14:763–766.
Article
35. Urga K, Narasimha HV. Phytate:zinc and phytate x calcium:zinc molar ratios in selected diets of Ethiopians. Bull Chem Soc Ethiop. 1998. 12:1–7.
36. Hurrell RF. Influence of vegetable protein sources on trace element and mineral bioavailability. J Nutr. 2003. 133:2973S–2977S.
Article
37. Mbithi-Mwikya S, Van Camp J, Mamiro PR, Ooghe W, Kolsteren P, Huyghebaert A. Evaluation of the nutritional characteristics of a finger millet based complementary food. J Agric Food Chem. 2002. 50:3030–3036.
Article
38. Berkman DS, Lescano AG, Gilman RH, Lopez SL, Black MM. Effects of stunting, diarrhoeal disease, and parasitic infection during infancy on cognition in late childhood: a follow-up study. Lancet. 2002. 359:564–571.
Article
39. Hamadani JD, Fuchs GJ, Osendarp SJ, Khatun F, Huda SN, Grantham-McGregor SM. Randomized controlled trial of the effect of zinc supplementation on the mental development of Bangladeshi infants. Am J Clin Nutr. 2001. 74:381–386.
Article
40. Dewey KG, Heinig MJ, Nommsen LA, Peerson JM, Lönnerdal B. Growth of breast-fed and formula-fed infants from 0 to 18 months: the DARLING Study. Pediatrics. 1992. 89:1035–1041.
Article
41. Umeta M, West CE, Haidar J, Deurenberg P, Hautvast JG. Zinc supplementation and stunted infants in Ethiopia: a randomised controlled trial. Lancet. 2000. 355:2021–2026.
Article
42. Rivera JA, Hotz C, González-Cossío T, Neufeld L, García-Guerra A. The effect of micronutrient deficiencies on child growth: a review of results from community-based supplementation trials. J Nutr. 2003. 133:4010S–4020S.
Article
43. Michaelsen KF, Hoppe C, Roos N, Kaestel P, Stougaard M, Lauritzen L, Mølgaard C, Girma T, Friis H. Choice of foods and ingredients for moderately malnourished children 6 months to 5 years of age. Food Nutr Bull. 2009. 30:S343–S404.
Article
44. Ijarotimi OS Jr, Olopade AJ. Determination of amino acid content and protein quality of complementary food produced from locally available food materials in ondo state, Nigeria. Malays J Nutr. 2009. 15:87–95.
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