Nutr Res Pract.  2012 Aug;6(4):277-285.

Antioxidant activities and determination of phenolic compounds isolated from oriental plums (Soldam, Oishiwase and Formosa)

• Kim SN
• Kim MR
• Cho SM
• Kim SY
• Kim JB
• Cho YS

Abstract

The purposes of this study were to determine phenolic compounds and to evaluate antioxidant activities of plums (Soldam, Oishiwase and Formosa). Soldam contains the highest amount of total phenolics among cultivars (Formosa: 4.0%, Oishiwase: 3.3%, Soldam: 6.4% for total phenolic) as well as the total flavonoids of which constituents were mainly myricetin and anthocyanidin. The antioxidant activities were measured by DPPH, ABTS radical scavenging, and SOD-like activities. The DPPH radical scavenging activity of Korean plum extracts (200 microg/mL) showed more than 43%, and the Soldam turned out to be the highest : ID50 value: 160-177 microg/mL for Formosa and Oishiwase; 58-64 microg/mL for Soldam. The ABTS radical scavenging activity of Korean plum extracts (200 microg/mL) was found to be more than 50%. The SOD-like activity of Korean plum extracts (200 microg/mL) showed more than 70%. Among three kinds of cultivars, Soldam had the highest antioxidant activity. The nitrite scavenging activity of Soldam was 61.5%, which is the highest, compared with that of the other cultivars, about 50%. From these results, Korean plums turned out to be phytochemical rich fruit as well as to show high antioxidant activities.

Keyword

Antioxidant activity; phenolic compounds; oriental plums

MeSH Terms

Anthocyanins
Benzothiazoles
Flavonoids
Fruit
Phenol
Prunus domestica
Sulfonic Acids
Taiwan
Anthocyanins
Benzothiazoles
Flavonoids
Phenol
Sulfonic Acids

Figure

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  Fig. 1 Total polyphenol (A) and flavonoid (B) contents of Korean plums (%, dry basis). Values are mean of three replicates. Different superscript in each column indicates the significant differences in the mean at P < 0.05.

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  Fig. 2 Chromatogram of flavonoid composition of standard compounds (A), Formosa (B), Oishiwase (C), and Soldam (D)

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  Fig. 3 Chromatogram of anthocyanin standard compounds (A), Formosa (B), Oishiwase (C), and Soldam (D)

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  Fig. 4 DPPH radical scavenging activity of BHT and Korean plum extracts. 1)Ultrasonification extracts at 60℃. 2)Shaking extracts at 60℃. 3)The BHT is used at the concentration of 25 µg/mL (n = 3). Different superscript in each column indicates the significant differences in the mean at P < 0.05.

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  Fig. 5 ABTS+ Scavenging activity of BHT and Korean plum extracts. 1)Ultrasonification extracts at 60℃. 2)Shaking extracts at 60℃. 3)The BHT is used at the concentration of 25 µg/mL (n = 3). Different superscript in each column indicates the significant differences in the mean at P < 0.05.

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  Fig. 6 SOD-like activity of BHT and Korean plum extracts. 1)Ultrasonification extracts at 60℃. 2)Shaking extracts at 60℃. 3)The BHT is used at the concentration of 25 µg/mL (n = 3). Different superscript in each column indicates the significant differences in the mean at P < 0.05.

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  Fig. 7 Nitrite scavenging of Korean plum extracts. 1)Ultrasonification extracts at 60℃. 2)Shaking extracts at 60℃. Values are mean of three replicates. Different superscript in each column indicates the significant differences in the mean at P < 0.05.

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