Nutr Res Pract.  2011 Aug;5(4):365-369.

Renoprotective and antioxidant effects of Saururus chinensis Baill in rats fed a high-fructose diet

• Choi HN
• Park YH
• Kim JH
• Kang MJ
• Jeong SM
• Kim HH
• Kim JI

Abstract

This study investigated the preventive effect of Saururus chinensis Baill against renal damage induced by a high-fructose diet in rats. The rats (n = 30) were fed either a cornstarch-based (65%), high-fructose (65%), or high-fructose (64.5%) diet with 0.5% S. chinensis Baill extract for 10 weeks. Twenty-four hour urine collections were obtained and the animals were sacrificed after an overnight fast. Serum urea and creatinine and urine albumin were measured using colorimetric methods, and creatinine clearance was determined. In addition, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and the activity of superoxide dismutase (SOD) in the kidney were determined. Kidney samples were also examined histologically. The fructose-fed rats showed renal dysfunction, indicated by decreased creatinine clearance, increased albumin in the urine, and increased urea and creatinine in the serum. These renal function parameters were comparable to control levels in rats that consumed S. chinensis Baill. Fructose consumption increased renal TBARS and reduced GSH and SOD activity, whereas these levels were near-normal in the rats consuming S. chinensis Baill. The kidneys of fructose-fed rats showed glomerular basement membrane thickening, mesangial matrix expansion, and tubule dilation. These pathological changes were not seen in the rats that consumed S. chinensis Baill. Therefore, S. chinensis Baill effectively alleviated fructose-induced renal damage in these rats, at least partially due to antioxidant activity.

Keyword

Saururus chinensis Baill; fructose; kidney; creatinine; antioxidant

MeSH Terms

Animals
Antioxidants
Creatinine
Diet
Fructose
Glomerular Basement Membrane
Glutathione
Kidney
Rats
Saururaceae
Superoxide Dismutase
Thiobarbiturates
Thiobarbituric Acid Reactive Substances
Urea
Urine Specimen Collection
Antioxidants
Creatinine
Fructose
Glutathione
Superoxide Dismutase
Thiobarbiturates
Thiobarbituric Acid Reactive Substances
Urea

Figure

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  Fig. 1 Renal histopathology of rats. Results of hematoxylin and eosin staining to characterize the morphological changes in rats fed diets containing 65% cornstarch (Control gorup), 65% fructose (Fructose group), or 64.5% fructose and 0.5% S. chinensis Baill extract (S. chinensis Baill group) for 10 weeks. (original magnification × 200)

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