Nat Prod Sci.  2015 Dec;21(4):251-254. 10.20307/nps.2015.21.4.251.

Anti-Inflammatory Effect of Violapyrones B and C from a Marine-derived Streptomyces sp.

• Lee HS
• An BJ
• Kim HJ
• Cho YH
• Kim DI
• Jang JY
• Kwak JH
• Lee HS
• Lee YJ
• Lee JS
• Shin HJ

• KMID: 2312905
• DOI: http://doi.org/10.20307/nps.2015.21.4.251

Abstract

Recently, we reported violapyrones B, C, H and I, unusual 3, 4, 6-trisubstituted alpha-pyrone derivatives, from the culture broth of the marine Streptomyces sp. 112CH148. In previous studies, violapyrones have been shown to have antibacterial and antitumor activities. However, the anti-inflammatory effect of violapyrones has not been reported yet. As part of our ongoing study for the discovery of bioactive metabolites from marine microorganisms, we found that violapyrones also have anti-inflammatory activity. In this study, we investigated the effect of violapyrones on LPS-induced inflammatory responses in vitro. Violapyrones B and C did not affect the viability of RAW 264.7 cells at concentrations up to 25 microM. However, violapyrones B and C inhibited the production of NO compared to the LPS-induced control. In addition, violapyrones B and C down-regulated the expression of iNOS protein in LPS-stimulated RAW 264.7 cells. To the best of our knowledge, this is the first report on the anti-inflammatory activity of violapyrones B and C.

Keyword

Violapyrones; alpha-Pyrones; Streptomyces sp.; Anti-inflammatory

MeSH Terms

Streptomyces*

Figure

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  Fig. 1. The structures of violapyrones B and C.

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  Fig. 2. Effect of violapyrones B and C on the cell viablility of RAW 264.7 cells.

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  Fig. 3. Effect of violapyrones B and C on the production of nitric oxide (NO) in LPS-induced macrophage RAW 264.7 cells.

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  Fig. 4. Effect of violapyrones B and C on the expression levels of iNOS and COX-2 proteins in LPS-stimulated macrophage RAW 264.7 cells.

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