Mycobiology.  2015 Sep;43(3):266-271. 10.5941/MYCO.2015.43.3.266.

Isolation and Identification of Yeasts from Wild Flowers Collected around Jangseong Lake in Jeollanam-do, Republic of Korea, and Characterization of the Unrecorded Yeast Bullera coprosmaensis

• Han SM
• Hyun SH
• Lee HB
• Lee HW
• Kim HK
• Lee JS

• KMID: 2312822
• DOI: http://doi.org/10.5941/MYCO.2015.43.3.266

Abstract

Several types of yeasts were isolated from wild flowers around Jangseong Lake in Jeollanam-do, Republic of Korea and identified by comparing the nucleotide sequences of the PCR amplicons for the D1/D2 variable domain of the 26S ribosomal DNA using Basic Local Alignment Search Tool (BLAST) analysis. In total, 60 strains from 18 species were isolated, and Pseudozyma spp. (27 strains), which included Pseudozyma rugulosa (7 strains) and Pseudozyma aphidis (6 strains), was dominant species. Among the 60 strains, Bullera coprosmaensis JS00600 represented a newly recorded yeast strain in Korea, and its microbiological characteristics were investigated. The yeast cell has an oval-shaped morphology measuring 1.4 x 1.7 microm in size. Bullera coprosmaensis JS00600 is an asporous yeast that exhibits no pseudomycelium formation. It grew well in vitamin-free medium as well as in yeast extract-malt extract broth and yeast extract-peptone-dextrose (YPD) broth, and it is halotolerant growing in 10% NaCl-containing YPD broth.

Keyword

Bullera coprosmaensis; Characteristics; Jangseong Lake; Unrecorded yeast; Wild flowers

MeSH Terms

Base Sequence
DNA, Ribosomal
Flowers*
Jeollanam-do*
Korea
Lakes*
Polymerase Chain Reaction
Republic of Korea*
Yeasts*
DNA, Ribosomal