Lab Anim Res.  2016 Mar;32(1):74-77. 10.5625/lar.2016.32.1.74.

Pathomorphological features of calcinosis circumscripta in a dog

• Lee EM
• Kim AY
• Lee EJ
• Jeong KS

• KMID: 2312157
• DOI: http://doi.org/10.5625/lar.2016.32.1.74

Abstract

A 19-month-old male German shepherd dog was presented with a hindlimb footpad mass. The mass was excised and histopathology was performed. Grossly, the cut section of the mass had multiple well-circumscribed nodules with a chalky appearance. Histopathologically, basophilic-stained calcium deposits of various sizes were distributed from dermis to subcutis, surrounded by epithelioid cells, multinucleated giant cells, and reactive fibroblasts. Myxoid metaplasia, calcium deposits in hair follicles, and psammoma-like bodies were also found. These histopathologic observations will greatly help to understand the pathogenesis of calcinosis circumscripta.

Keyword

Calcinosis circumscripta; histopathologic diagnosis; myxoid change; Psammoma-like body

MeSH Terms

Animals
Calcinosis*
Calcium
Dermis
Dogs*
Epithelioid Cells
Fibroblasts
Giant Cells
Hair Follicle
Hindlimb
Humans
Infant
Male
Metaplasia
Calcium

Figure

• 

  Figure 1 Gross findings. (A) A round mass protrudes from the footpad of the hindlimb. (B) Chalky calcified nodules of various sizes are observed in the cut section of the mass.

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  Figure 2 Microscopic findings of calcified nodules. (A) Basophilic-stained calcified nodules of various sizes are distributed from dermis to subcutis (original magnification 50×, scale bar=500 µm). (B, C) Calcification nodules are surrounded by epithelioid cells, multinucleated giant cells, and reactive fibroblasts (original magnifications 100× and 400×, scale bars= 200 and 50 µm).

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  Figure 3 Microscopic findings of other calcium deposits. (A, B) Hair follicles lack normal structure and have calcium deposits (original magnifications 100× and 400×, scale bars=200 and 50 µm). (C, D) Psammoma-like bodies are found within fibrous connective tissue (original magnifications 100× and 400×, scale bars=200 and 50 µm). (E, F) Basophilic-stained myxoid metaplasia is observed in the surrounding matrix tissue (original magnification 100×, scale bar=200 µm).

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