Lab Anim Res.  2016 Mar;32(1):1-7. 10.5625/lar.2016.32.1.1.

Postnatal changes in glucose transporter 3 expression in the dentate gyrus of the C57BL/6 mouse model

• Jung HY
• Yim HS
• Yoo DY
• Kim JW
• Chung JY
• Seong JK
• Yoon YS
• Kim DW
• Hwang IK

• KMID: 2312148
• DOI: http://doi.org/10.5625/lar.2016.32.1.1

Abstract

In this study, we observed the ontogenetic changes in glucose transporter 3 (GLUT3) immunoreactivity, a major neuronal GLUT, in the dentate gyrus of mouse brains at various ages: postnatal day (P) 1, 7, 14, 28, and 56. At P1, cresyl violet staining showed abundant neurons in the dentate gyrus, whereas the granule cell layer was ill-defined. At P7, the granule cell layer was observed, and cresyl violet-positive cells were dispersed throughout the polymorphic layer. At P14, the granule cell layer was well-defined, and cresyl violet positive cells were detected abundantly in the polymorphic layer. At P28 and P56, cresyl violet-positive cells were observed in the granule cell layer, as well as in the polymorphic layer. At P1, GLUT3 immunoreactivity was detected in the dentate gyrus. At P7, GLUT3 immunoreactive cells were scattered in the polymorphic and molecular layer. However, at P14, GLUT3 immunoreactivity was observed in the polymorphic layer as well as subgranular zone of the dentate gyrus. At P28, GLUT3 immunoreactivity was detected in the polymorphic layer of the dentate gyrus. At P56, GLUT3 immunoreactivity was observed predominantly in the subgranular zone of the dentate gyrus. GLUT3 immunoreactive cells were mainly colocalized with doublecortin, which is a marker for differentiated neuroblasts, in the polymorphic layer and subgranular zone of dentate gyrus at P14 and P56. These results suggest that the expression of GLUT3 is closely associated with postnatal development of the dentate gyrus and adult neurogenesis.

Keyword

Dentate gyrus; glucose transporter 3; mice; neuroblast; postnatal development

MeSH Terms

Adult
Animals
Brain
Dentate Gyrus*
Glucose Transport Proteins, Facilitative*
Glucose*
Humans
Mice*
Neurogenesis
Neurons
Viola
Glucose
Glucose Transport Proteins, Facilitative

Figure

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  Figure 1 Cresyl violet staining in the mouse dentate gyrus at P1 (A), P7 (B), P14 (C), P28 (D), and P56 (E). Cresyl violet-positive neurons are dispersed throughout the dentate gyrus by P7. Cresyl violet-positive neurons are detected in the granule cell layer (GCL) and well-laminated into three layers at P14. ML, molecular layer; PL, polymorphic layer. Scale bar=50 µm.

• 

  Figure 2 Immunohistochemical staining for glucose transporter 3 (GLUT3) in the mouse dentate gyrus at P1 (A), P7 (B), P14 (C), P28 (D), and P56 (E). GLUT3 immunoreactivity is detected in the cell body and neuropils of the polymorphic (PL) and granule cell layers (GCL) of the dentate gyrus at P14. In the P28 and P56 groups, GLUT3 immunoreactivity is mainly observed in the subgranular zone of the dentate gyrus. ML, molecular layer. Scale bar=50 µm. F and G: The number of GLUT3 immunoreactive cells and relative optical density (ROD) of GLUT3 immunoreactivity in the dentate gyrus at P1, P7, P14, P28, and P56 are expressed as percentage of P1 group (n=5 per group; aP<0.05, significantly different from the P1 group; bP<0.05, significantly different from the P7 group; cP<0.05, significantly different from the P14 group; dP<0.05, significantly different from the P28 group). All data are represented as the mean±standard errors of mean.

• 

  Figure 3 Immunofluorescence staining for GLUT3 (A and D, red), doublecortin (DCX, B and E, green), and merged images (C and F, yellow) in the dentate gyrus in the P14 and P56 groups. Note that GLUT3 and DCX double-labeled cells are abundant in the polymorphic layer of the dentate gyrus in the P14 group, while in the P56 group these cells are mainly detected in the subgranular zone of the dentate gyrus. Scale bar=50 µm.

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