Lab Anim Res.  2013 Mar;29(1):27-32. 10.5625/lar.2013.29.1.27.

Usefulness of a Helicobacter pylori stool antigen test for diagnosing H. pylori infected C57BL/6 mice

• Moon DI
• Shin EH
• Oh HG
• Oh JS
• Hong S
• Chung Y
• Kim O

• KMID: 2312100
• DOI: http://doi.org/10.5625/lar.2013.29.1.27

Abstract

Among several diagnostic tests, a Helicobacter pylori stool antigen (HpSA) test may offer a useful noninvasive method for diagnosing infection without sacrificing animals. In this study, male C57BL/6 mice (n=6) were infected with H. pylori ATCC 49503 (1x10(8) CFU/mouse) by intragastric inoculation three times at 2-day intervals, and H. pylori infected stool specimens were collected 1, 3, 5, 7, 14, 21 days after infection to assess reliability of the HpSA test. Five of six specimens were positive at 5-21 days after infection, and the sensitivity of the HpSA test was 83.33%. The presence of H. pylori infection was confirmed by the rapid urease test and genomic DNA polymerase chain reaction (PCR), and showed the same results as the HpSA. However, the rapid urease test and genomic DNA PCR are invasive tests and require animal sacrifice to detect H. pylori in gastric biopsy samples. We suggest that an HpSA test kit would be useful and effective for monitoring H. pylori in various laboratory animals, as H. pylori can be easily monitored without sacrificing animals.

Keyword

Helicobacter pylori; H. pylori stool antigen KIT; non-invasive; CLO; HpSA

MeSH Terms

Animals
Animals, Laboratory
Biopsy
Diagnostic Tests, Routine
DNA
Helicobacter
Helicobacter pylori
Humans
Male
Mice
Polymerase Chain Reaction
Urease
DNA
Urease

Figure

• 

  Figure 1 Results of Helicobacter pylori Stool Ag Kit based on the number of H. pylori ATCC 49503 cells. (A) 1×105 CFU/100 µL; (B) 1×106 CFU/100 µL; (C) 1×107 CFU/100 µL; (D) 1×108 CFU/100 µL.

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