Int Neurourol J.  2016 May;20(Suppl 1):S15-S22.

See-Through Technology for Biological Tissue: 3-Dimensional Visualization of Macromolecules

• Lee E
• Kim HJ
• Sun W

Abstract

Tissue clearing technology is currently one of the fastest growing fields in biomedical sciences. Tissue clearing techniques have become a powerful approach to understand further the structural information of intact biological tissues. Moreover, technological improvements in tissue clearing and optics allowed the visualization of neural network in the whole brain tissue with subcellular resolution. Here, we described an overview of various tissue-clearing techniques, with focus on the tissue-hydrogel mediated clearing methods, and discussed the main advantages and limitations of transparent tissue for clinical diagnosis.

Keyword

Optical Tissue Clearing; Imaging, Three-Dimensional; Immunolabeling; Neuroscience; Molecular Diagnosis

MeSH Terms

Brain
Diagnosis
Imaging, Three-Dimensional
Neurosciences