Bactericidal Effect of Electrolyzed Oxidized Water Generated by WATRIX(R)-10G against Clinical Isolates

Choi, Tae Youn; Lee, Ka Yeon

Abstract

Background: Disinfection is essential for the prevention of hospital infection. The electrolyzed oxidized water generated by WATRIX(R)-10G(Microbank co., Korea) was recently developed as a disinfectant. This is not costly and does not cause any clinical problems and environmental pollution. We evaluated bactericidal activity of the electrolyzed oxidized water generated by WATRIX(R)-10G against several clinical isolates of bacteria and yeasts.
Methods
23 strains of bacteria and four strains of yeast were exposed to the electrolyzed oxidized water(40&50ppm HOel) generated by WATRIX(R)-10G for the various periods (0.5, 1, 2, 4, 10, 15, 30, 60minutes). After the exposure to electrolyzed oxidized water, 0.01mL of mixture of microorganisms and electrolyzed oxidized water was inoculated into brain-heart infusion broth or onto Sabouraud dextrose agar and incubated at 35degrees C for 48 hours.
Results
All strains of bacteria and yeast were killed within 30 seconds after an exposure to 40 ppm of electrolyzed oxidized water. All vegetative forms of Bacillus subtills were killed within 10, 4 and 2 minutes in 30, 40 and 50 ppm of electrolyzed oxidized water. respectively. But, all spore forms of Bacillus subtilis were killed within 30, 15 and 10 minutes in 30, 40 and 50 ppm of electrolyzed oxidized water respectively.
Conclusions
This study showed that the electrolyzed oxidized water generated by WATRIX(R)-10G was effective not only for the disinfection of commonly isolated bacteria and yeast from hospital but also against spore-forming bacteria. It may be recommended that WATRIX(R)-10G should be used for the effective disinfection of skin, instruments ,hospital floors and hand-washing.