Korean J Nosocomial Infect Control.
2007 Dec;12(2):77-84.
Prevalence of vanA Vancomycin-Resistant Enterococci and Epidemiologic Survey of Chicken Farms Located in Seoul and Kyunggi Province
- Affiliations
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- 1Graduate School of Public Health, College of Medicine, Korea University, Korea.
- 2Department of Preventive Medicine, College of Medicine, Korea University, Korea. kimsd@korea.ac.kr
- 3Department of Biostatistics Medicine, College of Medicine, Korea University, Korea.
- 4Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.
Abstract
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BACKGROUND: Avoparcin has been banned in Korea since 8 years ago, but vancomycin-resistant enterococci (VRE) has not been proven to be eradicated from the domestic livestock. This study was purposed to investigate the prevalence of VRE and perform an epidemiologic survey in chicken farms located in Seoul and Kyunggi Province.
METHODS
Feces were collected freshly from chickens and workers and their families in three free-range chicken farms and three cage-raising chicken farms. All swabs were inoculated on enterococcosel agar containing 6 microgram/mL of vancomycin. Minimum inhibitory concentrations of teicoplanin and vancomycin were determined using the agar dilution method. Vancomycin resistance were genotyped with multiplex PCR using primers specific for vanA, vanB, and vanC1. Epidemiolgic survey was carried out using a uniform questionnaire to obtain information about the number and variety of livestock, feeding protocols, physical farming conditions, maintenance protocols, hygiene, and employment conditions.
RESULTS
vanA Enterococcus facium was isolated from 37 (2.9%) of 1280 chickens. There were no VRE carriers found among the workers and their families. The prevalence of VRE among cage-raising chickens was 4.8% compared to 0.6% for free-range chickens. The difference between the two groups was highly significant (P<0.001).
CONCLUSION
There still was a significant rate of VRE colonization in chicken livestock in Seoul and Kyunggi Province. Physical farming conditions of cage-raising chicken farms seemed to be associated with a high VRE colonization rate.
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