Korean J Blood Transfus.
2009 Dec;20(3):247-252.
Chimerism Proven by Flow Cytomety in the Setting of an ABO Discrepancy: A Case Report
- Affiliations
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- 1Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea. dcho@chonnam.ac.kr
- 2Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.
Abstract
- Chimerism is an important, yet uncommon cause of ABO phenotype/genotype discrepancies. The propositus was a 28 year-old pregnant women who had an ABO discrepancy, expressing an A(weak)B RBC phenotype and AB reverse type. Sequencing of ABO exons 6 and 7 revealed a B101/O01 genotype. Neither analysis of 9 short tandem repeats (STR) loci nor HLA typing on DNA extracted from white blood cells demonstrated evidence of chimerism. However, flow cytometric analysis using a PE-conjugated anti-A mouse monoclonal antibody detected a small population (3.8%) of red cells expressing normal A antigen. Based on this, we suggest that flow cytometric analysis is an effective method for the identification of small chimeric populations of RBCs in the immunohematology laboratory.
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