Korean J Blood Transfus.  2013 Dec;24(3):233-240.

Clinical Significance of Antibodies Against Platelet HLA Class I in Children with Idiopathic Thrombocytopenic Purpura

• Lee HJ
• Yeom JS
• Park JS
• Park ES
• Seo JH
• Lim JY
• Park CH
• Woo HO
• Youn HS

Abstract

BACKGROUND
A previous history of transfusion has been known to be associated with production of anti-HLA class I antibodies. However, platelet glycoproteins are the main target of idiopathic thrombocytopenic purpura (ITP). The mechanism of antibody production is known to differ significantly between glycoproteins and anti-HLA class I. The aim of this study was to evaluate the clinical significance of anti-HLA class I antibodies in childhood ITP.
METHODS
Enrollment for the normal control group targeted 48 people who visited Gyeongsang National University Hospital from 1990 to 2010, and 48 young children with ITP. Anti-glycoproteins and anti-HLA class I antibodies were tested using the Modified Antigen Capture Enzyme-linked immunosorbent assay (MACE) kit.
RESULTS
The positive rate of anti-HLA antibodies was significantly different [36/39 (92.3%) vs 29/46 (63%)] [ITP group vs normal control group] (P=0.002). The mean positive S/C ratio of anti-HLA antibodies was also significantly different (3.55 vs 1.51) [ITP group vs normal control group] (P=0.0000). The positive rate of anti-HLA did not differ significantly between the transfused group and the non-transfused group [12/12 (100%) vs 24/27 (88%)] [transfused ITP vs non-transfused ITP]. The mean positive S/C ratio of anti-HLA antibodies did not differ significantly between the transfused ITP group and the non-transfused ITP group (4.30 vs 3.25) [transfused ITP vs non-transfused ITP]. Consecutive testing showed that positive rate and positive S/C ratio of anti-HLA antibodies did not change significantly between sampling times in both groups [transfused ITP vs non-transfused ITP] (P=1.00 and P=0.15).
CONCLUSION
Anti-HLA class I antibodies may be involved in childhood ITP. Transfusion did not affect the course of childhood ITP.

Keyword

ITP (idiopathic thrombocytopenia purpura); HLA class I; GP (glycoprotein)

MeSH Terms

Antibodies*
Antibody Formation
Blood Platelets*
Child*
Enzyme-Linked Immunosorbent Assay
Glycoproteins
Humans
Platelet Membrane Glycoproteins
Purpura, Thrombocytopenic, Idiopathic*
Antibodies
Glycoproteins
Platelet Membrane Glycoproteins