Korean J Asthma Allergy Clin Immunol.  2011 Jun;31(2):131-139.

Effects of CpG Oligodeoxynucleotide on Airway Inflammation after Topical Application of and Airway Challenge with Dermatophagoides farinae in NC/Nga Mice

Abstract

BACKGROUND
Atopic dermatitis (AD) is the initial step of allergic march that progresses to airway allergic disease. There has been much interest in the interruption of the allergic march, but the results are not promising. CpG oligodeoxynucleotide (ODN) has been reported to have both preventive and therapeutic effects on the development of asthma in mouse models.
OBJECTIVE
To identify the development of airway inflammation after having atopic dermatitis-like skin lesions induced by topical application of Dermatophagoides farinae (Df), and to evaluate the preventive effect of CpG ODN on the development of airway inflammation. METHOD: We injected 5microg of Df intradermally 8 times for 2 weeks into 8-week-old NC/Nga mice and then challenged them with 3 times of Df intranasally. CpG ODN with or without Df was injected subcutaneously before airway challenge. Airway hyperreactivity was determined by the change inenhanced pause (Penh) during methacholine provocation. Lung tissue and bronchoalveolar lavage specimens were evaluated. RESULT: Topical Df application induced AD-like skin lesion microscopically, but not macroscopically. NC/Nga mice with topical Df application developed more severe airway inflammation upon intranasal Df challenge than those with topical PBS application. However, the mice with CpG ODN injection before airway challenge showed reduced airway inflammation. The levels of Df-specific IgG1 were increased in the mice with both topical Df application and intranasal Df challenge but were decreased in the mice with CpG ODN injection.
CONCLUSION
These results suggest that CpG may inhibit the development of airway inflammation from those with previous AD-like skin lesions induced by Df.

MeSH Terms

Animals
Asthma
Bronchoalveolar Lavage
Dermatitis, Atopic
Dermatophagoides farinae
Immunoglobulin G
Inflammation
Lung
Methacholine Chloride
Mice
Pyroglyphidae
Skin
Immunoglobulin G
Methacholine Chloride