Korean J Anesthesiol.  2010 Apr;58(4):378-382. 10.4097/kjae.2010.58.4.378.

Propofol has delayed myocardial protective effects after a regional ischemia/reperfusion injury in an in vivo rat heart model

• Shin IW
• Jang IS
• Lee SH
• Baik JS
• Park KE
• Sohn JT
• Lee HK
• Chung YK

• KMID: 2299337
• DOI: http://doi.org/10.4097/kjae.2010.58.4.378

Abstract

BACKGROUND
It is well known that propofol protects myocardium against myocardial ischemia/reperfusion injury in the rat heart model. The aim of this study was to investigate whether propofol provides a protective effect against a regional myocardial ischemia/reperfusion injury in an in vivo rat heart model after 48 h of reperfusion.
METHODS
Rats were subjected to 25 min of left coronary artery occlusion followed by 48 h of reperfusion. The sham group received profopol without ischemic injury. The control group received normal saline with ischemia/reperfusion injury. The propofol group received profopol with ischemia/reperfusion injury. The intralipid group received intralipid with ischemia/reperfusion injury. A microcatheter was advanced into the left ventricle and the hemodynamic function was evaluated. The infarct size was determined by triphenyltetrazolium staining. The serum level of cardiac troponin-I (cTn-I) was determined by ELISA (enzyme-linked immunosorbent assay).
RESULTS
Propofol demonstrated protective effects on hemodynamic function and infarct size reduction. In the propofol group, the +dP/dt(max) (P = 0.002) was significantly improved compared to the control group. The infarct size was 49.8% of the area at risk in the control group, and was reduced markedly by administration of propofol to 32.6% in the propofol group (P = 0.014). The ischemia/reperfusion-induced serum level of cTn-I was reduced by propofol infusion during the peri-ischemic period (P = 0.0001).
CONCLUSIONS
Propofol, which infused at clinically relevant concentration during the peri-ischemic period, has delayed myocardial protective effect after regional myocardial ischemia/reperfusion injury in an in vivo rat heart model after 48 h of reperfusion.

Keyword

Ischemia/reperfusion injury; Myocardium; Propofol

MeSH Terms

Animals
Coronary Vessels
Emulsions
Enzyme-Linked Immunosorbent Assay
Heart
Heart Ventricles
Hemodynamics
Myocardium
Phospholipids
Propofol
Rats
Reperfusion
Salicylamides
Soybean Oil
Tetrazolium Salts
Troponin I
Emulsions
Phospholipids
Propofol
Salicylamides
Soybean Oil
Tetrazolium Salts
Troponin I